RT info:eu-repo/semantics/article
T1 Differential membrane lipid profiles and vibrational spectra of three edaphic algae and one cyanobacterium
A1 Montero, Olimpio
A1 Velasco, Marta
A1 Miñón, Jorge
A1 Marks, Evan A. N.
A1 Sanz Arranz, José Aurelio
A1 Rad, Carlos
K1 Algas
K1 Acuicultura
K1 Soil algae
K1 UPLC–MS
K1 Oocystis
K1 Microcoleus
K1 Algas del suelo
K1 UPLC–EM
K1 Oocistis
K1 Microcoleo
K1 2302.21 Biología Molecular
K1 2409.92 Genética Molecular de Plantas
AB The membrane glycerolipids of four phototrophs that were isolated from an edaphic assemblage were determined by UPLC–MS after cultivation in a laboratory growth chamber. Identification was carried out by 18S and 16S rDNA sequencing. The algal species were Klebsormidium flaccidum (Charophyta), Oocystis sp. (Chlorophyta), and Haslea spicula (Bacillariophyta), and the cyanobacterium was Microcoleus vaginatus (Cyanobacteria). The glycerolipid profile of Oocystis sp. was dominated by monogalactosyldiacylglycerol (MGDG) species, with MGDG(18:3/16:4) accounting for 68.6%, whereas MGDG(18:3/16:3) was the most abundant glycerolipid in K. flaccidum (50.1%). A ratio of digalactosyldiacylglycerol (DGDG) species to MGDG species (DGDG/MGDG) was shown to be higher in K. flaccidum (0.26) than in Oocystis sp. (0.14). This ratio increased under high light (HL) as compared to low light (LL) in all the organisms, with its highest value being shown in cyanobacterium (0.38–0.58, LL−HL). High contents of eicosapentaenoic acid (EPA, C20:5) and hexadecenoic acid were observed in the glycerolipids of H. spicula. Similar Fourier transform infrared (FTIR) and Raman spectra were found for K. flaccidum and Oocystis sp. Specific bands at 1629.06 and 1582.78 cm−1 were shown by M. vaginatus in the Raman spectra. Conversely, specific bands in the FTIR spectrum were observed for H. spicula at 1143 and 1744 cm−1. The results of this study point out differences in the membrane lipid composition between species, which likely reflects their different morphology and evolutionary patterns.
PB MDPI
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/59419
UL https://uvadoc.uva.es/handle/10324/59419
LA eng
NO International Journal Molecular Science, 2021, vol. 22, n. 20, 11277
NO Producción Científica
DS UVaDOC
RD 17-jul-2024