RT info:eu-repo/semantics/article
T1 Ocular surface pathology in patients suffering from mercury intoxication
A1 Cañadas Suárez, María del Pilar
A1 Lantigua Dorville, Yrbani
A1 Enriquez De Salamanca Aladro, Amalia
A1 Fernández Martínez, Itziar
A1 Pastor Idoate, Salvador
A1 Sobas Abad, Eva María
A1 Dueñas Laita, Antonio
A1 Pérez Castrillon, José Luis
A1 Pastor Jimeno, José Carlos
A1 Calonge, Margarita
K1 Ophthalmology
K1 Ojos - Enfermedades
K1 Eye - Diseases
K1 Cornea - Diseases - Diagnosis
K1 Confocal microscopy
K1 Cytokines
K1 Dry eye syndromes
K1 Mercury poisoning
K1 Tear biomarkers
K1 3201.09 Oftalmología
K1 3214 Toxicología
AB Purpose: To report the ocular surface pathology of patients suffering from acute/subacute mercury vapor intoxication. Design: Cross-sectional study. Participants: Male workers intoxicated with inorganic mercury referred for ophthalmic involvement and healthy control subjects. Methods: The following tests were performed: dry eye (DE)-related symptoms indicated by the ocular surface disease (OSDI) index questionnaire; tear osmolarity; analysis of 23 tear cytokine concentrations and principal component and hierarchical agglomerative cluster analyses; tear break-up time (T-BUT); corneal fluorescein and conjunctival lissamine green staining; tear production by Schirmer and tear lysozyme tests; mechanical and thermal corneal sensitivity (non-contact esthesiometry); and corneal nerve analysis and dendritic cell density by in vivo confocal microscopy (IVCM). Results: Twenty-two out of 29 evaluated patients entered the study. Most had DE-related symptoms (OSDI values > 12), that were severe in 63.6% of them. Tear osmolarity was elevated (>308 mOsms/L) in 83.4% of patients (mean 336.23 (28.71) mOsm/L). Corneal and conjunctival staining were unremarkable. T-BUT was low (<7 s) in 22.7% of patients. Schirmer test and tear lysozyme concentration were low in 13.6% and 27.3% of cases, respectively. Corneal esthesiometry showed patient mechanical (mean 147.81 (53.36) mL/min) and thermal thresholds to heat (+2.35 (+1.10) °C) and cold (−2.57 (−1.24) °C) to be significantly higher than controls. Corneal IVCM revealed lower values for nerve density (6.4 (2.94) n/mm2), nerve branching density (2 (2.50) n/mm2), and dendritic cell density (9.1 (8.84) n/mm2) in patients. Tear levels of IL-12p70, IL-6, RANTES, and VEGF were increased, whereas EGF and IP-10/CXCL10 were decreased compared to controls. Based on cytokine levels, two clusters of patients were identified. Compared to Cluster 1, Cluster 2 patients had significantly increased tear levels of 18 cytokines, decreased tear lysozyme, lower nerve branching density, fewer dendritic cells, and higher urine mercury levels. Conclusions: Patients suffering from systemic mercury intoxication showed symptoms and signs of ocular surface pathology, mainly by targeting the trigeminal nerve, as shown by alterations in corneal sensitivity and sub-basal nerve morphology.
PB MDPI
SN 2075-4418
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/59498
UL https://uvadoc.uva.es/handle/10324/59498
LA eng
NO Diagnostics, 2021, Vol. 11, Nº. 8, 1326
NO Producción Científica
DS UVaDOC
RD 22-nov-2024