RT info:eu-repo/semantics/article
T1 Use of aequorin-based indicators for monitoring Ca2+ in acidic organelles
A1 Alonso Alonso, María Teresa
A1 Torres Vidal, Patricia
A1 Calvo Rodríguez, Belén
A1 Rodriguez, C
A1 Río Lorenzo, Alba del
A1 Rojo Ruiz, Jonatan
A1 García-Sancho Martín, Francisco Javier
A1 Patel, S
K1 Biología celular
K1 Biochemistry
K1 Subcellular Ca2+
K1 Organelle
K1 Luminescence
K1 Acidic
K1 Ca2+ subcelular
K1 Orgánulo
K1 Luminiscencia
K1 Ácido
K1 2302.21 Biología Molecular
K1 2407 Biología Celular
AB Over the last years, there is accumulating evidence that acidic organelles can accumulate and release Ca2+ upon cell activation. Hence, reliable recording of Ca2+ dynamics in these compartments is essential for understanding the physiopathological aspects of acidic organelles. Genetically encoded Ca2+ indicators (GECIs) are valuable tools to monitor Ca2+ in specific locations, although their use in acidic compartments is challenging due to the pH sensitivity of most available fluorescent GECIs. By contrast, bioluminescent GECIs have a combination of features (marginal pH sensitivity, low background, no phototoxicity, no photobleaching, high dynamic range and tunable affinity) that render them advantageous to achieve an enhanced signal-to-noise ratio in acidic compartments. This article reviews the use of bioluminescent aequorin-based GECIs targeted to acidic compartments. A need for more measurements in highly acidic compartments is identified.
PB Elsevier
SN 0167-4889
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/59558
UL https://uvadoc.uva.es/handle/10324/59558
LA eng
NO Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2023, vol. 1870, Issue 6, 119481
NO Producción Científica
DS UVaDOC
RD 12-sep-2024