RT info:eu-repo/semantics/article T1 Biotic and abiotic insights into the storage of food waste and its effect on biohydrogen and methane production potential A1 García Depraect, Octavio A1 Mirzazada, Inji A1 Martínez Mendoza, Leonardo José A1 Regueira Marcos, Lois A1 Muñoz Torre, Raúl K1 Microbiology K1 Biochemistry K1 Biogas K1 Hydrogen K1 Lactate K1 Storage K1 Biogás K1 Hidrógeno K1 Lactato K1 Almacenamiento K1 3303.03 Procesos Químicos K1 2414 Microbiología AB The present study investigated the physicochemical and microbiological changes occurring during the storage of simulated restaurant food waste (FW) and how such changes affected its biohydrogen and biogas production potential. FW was stored for 72 h in a closed atmosphere under two different scenarios: i) without and ii) with inoculation of a mixed microbial culture harboring lactic acid bacteria (LAB). Both storage scenarios resulted in similar biotic and abiotic changes in FW. Particularly, FW was pre-acidified and pre-hydrolyzed to some extent during the storage, resulting in a feedstock enriched in LAB (≈ 95 % total relative abundance) and lactate (10.5–12.3 g/L, 87.0–90.5 % selectivity). Biochemical hydrogen potential tests revealed that the use of stored FW resulted in similar or even higher hydrogen production efficiencies compared to that of non-stored FW, achieving up to 60 NmL H2/g VS added and a maximum volumetric hydrogen production rate of 9.7 NL H2/L-d. Metabolically, the conversion of lactate into hydrogen was crucial regardless of the use of non-stored or stored FW, albeit the presence of fermentable carbohydrates in the substrate was also essential either to produce lactate or to co-produce extra hydrogen. On the contrary, biochemical methane potential tests showed that the biogas production potential of FW was not affected by storage, yielding on average 400 NmL CH4/g VS added and revealing that lactate oxidation to methane precursors represented an important step in FW biomethanization. PB Elsevier SN 2214-7144 YR 2023 FD 2023 LK https://uvadoc.uva.es/handle/10324/59685 UL https://uvadoc.uva.es/handle/10324/59685 LA eng NO Journal of Water Process Engineering, 2023, vol. 53, 103840 NO Producción Científica DS UVaDOC RD 28-nov-2024