RT info:eu-repo/semantics/article T1 Dual RNA-sequencing analysis of resistant (Pinus pinea) and susceptible (Pinus radiata) hosts during Fusarium circinatum challenge A1 Zamora Ballesteros, Cristina A1 Pinto, Gloria A1 Amaral, Joana A1 Valledor, Luis A1 Alves, Artur A1 Díez Casero, Julio Javier A1 Martín García, Jorge K1 Pinos - Enfermedades y plagas K1 Hongos patógenos K1 Pinos K1 Host-parasite relationships K1 Pathogenic microorganisms K1 Bosques y silvicultura K1 3106 Ciencia Forestal K1 3106.08 Silvicultura K1 3103.08 Gestión de la Producción Vegetal AB Fusarium circinatum causes one of the most important diseases of conifers worldwide, the pine pitch canker (PPC). However, no effective field intervention measures aiming to control or eradicate PPC are available. Due to the variation in host genetic resistance, the development of resistant varieties is postulated as a viable and promising strategy. By using an integrated approach, this study aimed to identify differences in the molecular responses and physiological traits of the highly susceptible Pinus radiata and the highly resistant Pinus pinea to F. circinatum at an early stage of infection. Dual RNA-Seq analysis also allowed to evaluate pathogen behavior when infecting each pine species. No significant changes in the physiological analysis were found upon pathogen infection, although transcriptional reprogramming was observed mainly in the resistant species. The transcriptome profiling of P. pinea revealed an early perception of the pathogen infection together with a strong and coordinated defense activation through the reinforcement and lignification of the cell wall, the antioxidant activity, the induction of PR genes, and the biosynthesis of defense hormones. On the contrary, P. radiata had a weaker response, possibly due to impaired perception of the fungal infection that led to a reduced downstream defense signaling. Fusarium circinatum showed a different transcriptomic profile depending on the pine species being infected. While in P. pinea, the pathogen focused on the degradation of plant cell walls, active uptake of the plant nutrients was showed in P. radiata. These findings present useful knowledge for the development of breeding programs to manage PPC. PB MDPI SN 1422-0067 YR 2021 FD 2021 LK https://uvadoc.uva.es/handle/10324/59723 UL https://uvadoc.uva.es/handle/10324/59723 LA eng NO International Journal of Molecular Sciences, 2021, Vol. 22, Nº. 10, 5231 NO Producción Científica DS UVaDOC RD 15-ene-2025