RT info:eu-repo/semantics/article
T1 Kirkiin: A new toxic type 2 ribosome-Inactivating protein from the caudex of Adenia kirkii
A1 Bortolotti, Massimo
A1 Maiello, Stefania
A1 Ferreras Rodríguez, José Miguel
A1 Iglesias Álvarez, María del Rosario
A1 Polito, Letizia
A1 Bolognesi, Andrea
K1 Plant toxins
K1 Toxinas
K1 Proteins - Synthesis
K1 Proteínas
K1 Ribosomes - Structure
K1 Apoptosis
K1 Lectins
K1 Neuroblastoma
K1 Pharmacology/Toxicology
K1 Ribosome-inactivating protein
K1 3209 Farmacología
K1 3214 Toxicología
K1 2302 Bioquímica
AB Ribosome-inactivating proteins (RIPs) are plant toxins that irreversibly damage ribosomes and other substrates, thus causing cell death. RIPs are classified in type 1 RIPs, single-chain enzymatic proteins, and type 2 RIPs, consisting of active A chains, similar to type 1 RIPs, linked to lectin B chains, which enable the rapid internalization of the toxin into the cell. For this reason, many type 2 RIPs are very cytotoxic, ricin, volkensin and stenodactylin being the most toxic ones. From the caudex of Adenia kirkii (Mast.) Engl., a new type 2 RIP, named kirkiin, was purified by affinity chromatography on acid-treated Sepharose CL-6B and gel filtration. The lectin, with molecular weight of about 58 kDa, agglutinated erythrocytes and inhibited protein synthesis in a cell-free system at very low concentrations. Moreover, kirkiin was able to depurinate mammalian and yeast ribosomes, but it showed little or no activity on other nucleotide substrates. In neuroblastoma cells, kirkiin inhibited protein synthesis and induced apoptosis at doses in the pM range. The biological characteristics of kirkiin make this protein a potential candidate for several experimental pharmacological applications both alone for local treatments and as component of immunoconjugates for systemic targeting in neurodegenerative studies and cancer therapy.
PB MDPI
SN 2072-6651
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/59905
UL https://uvadoc.uva.es/handle/10324/59905
LA eng
NO Toxins, 2021, Vol. 13, Nº. 2, 81
NO Producción Científica
DS UVaDOC
RD 24-nov-2024