RT info:eu-repo/semantics/article
T1 Molecular evolution of epididymal lipocalin genes localized on mouse chromosome 2
A1 Suzuki, Kichiya
A1 Lareyre, Jean-Jacques
A1 Sánchez Romero, Diego
A1 Gutiérrez, Gabriel
A1 Araki, Yoshihiko
A1 Matusik, Robert J.
A1 Orgebin Crist, Marie Claire
K1 Lipocainas
K1 Genética
K1 Fecundidad femenina
AB We previously identified two murine secretory proteins, mE-RABP(Lcn5) and mEP17(Lcn8), belonging to the lipocalin family andspecifically expressed in the epididymis. The genes are contiguous and localized on mouse chromosome 2. We now show that five otherrelated lipocalin genes, Lcn9, Lcn10, Lcn11, Lcn12, and Lcn13, that evolved by in situ tandem duplication are present on the same locus.Lcn9, Lcn10, Lcn12, and Lcn13 genes, like Lcn5 and Lcn8 genes, are specifically expressed in the mouse epididymis. However, each genehas a distinct spatial expression within the epididymis and different regulation. Analysis of the human genome sequence shows the presenceof genes encoding lipocalins with genomic organization, chromosomal arrangement, and orientation similar to that of the correspondingmurine genes, indicating that the epididymal cluster is evolutionary conserved. A phylogenetic analysis of the new epididymal proteinsreveals their spread position in the lipocalin protein family tree. This suggests the preservation of the regulatory sequences, while proteinsequences have greatly diverged, reflecting functional diversity and possibly multifunctionality. In terms of the cluster ancestry, epididymalexpression possibly appeared in a PGDS-like lipocalin in amniotes, and the duplications generating the cluster occurred at least in thecommon ancestor of rodents and primates. The presence and conservation of a cluster of five genes encoding epididymal lipocalins,differently regulated and regionalized in the epididymis, strongly suggests that these proteins may play an important role for male fertility.
PB Elsevier
SN 0378-1119
YR 2004
FD 2004
LK http://uvadoc.uva.es/handle/10324/6082
UL http://uvadoc.uva.es/handle/10324/6082
LA eng
NO Gene, 2004, vol. 339, p. 49-59
NO Producción Científica
DS UVaDOC
RD 03-mar-2025