RT info:eu-repo/semantics/article
T1 ApoD, a Glia-Derived Apolipoprotein, Is Required for Peripheral Nerve Functional Integrity and a Timely Response to Injury
A1 Ganfornina Álvarez, María Dolores
A1 Carmo, Sonia do
A1 Martínez, Eva
A1 Tolivia Fernández, Jorge Luis
A1 Navarro, Ana
A1 Rassart, Eric
A1 Sánchez Romero, Diego
K1 Células reproductoras
K1 Lipocainas
AB Glial cells are a key element to the process of axonal regeneration,either promoting or inhibiting axonal growth. The studyof glial derived factors induced by injury is important tounderstand the processes that allow or preclude regeneration,and can explain why the PNS has a remarkable ability toregenerate, while the CNS does not. In this work we focus onApolipoprotein D (ApoD), a Lipocalin expressed by glial cellsin the PNS and CNS. ApoD expression is strongly inducedupon PNS injury, but its role has not been elucidated. Here weshow that ApoD is required for: (1) the maintenance of peripheralnerve function and tissue homeostasis with age, and (2)an adequate and timely response to injury. We study crushedsciatic nerves at two ages using ApoD knock-out and transgenicmice over-expressing human ApoD. The lack of ApoDdecreases motor nerve conduction velocity and the thicknessof myelin sheath in intact nerves. Following injury, we analyzethe functional recovery, the cellular processes, and the proteinand mRNA expression profiles of a group of injury-inducedgenes. ApoD helps to recover locomotor function after injury,promoting myelin clearance, and regulating the extent ofangiogenesis and the number of macrophages recruited to theinjury site. Axon regeneration and remyelination are delayedwithout ApoD and stimulated by excess ApoD. The mRNA andprotein expression profiles reveal that ApoD is functionallyconnected in an age-dependent manner to specific molecularprograms triggered by injury.
PB Wiley-Liss
SN 0894-1491
YR 2010
FD 2010
LK http://uvadoc.uva.es/handle/10324/6084
UL http://uvadoc.uva.es/handle/10324/6084
LA eng
NO Glia, 2010, vol. 58, p. 1320-1334
NO Producción Científica
DS UVaDOC
RD 20-abr-2024