RT info:eu-repo/semantics/article
T1 Exon-Intron Structure and Evolution of the Lipocalin Gene Family
A1 Sánchez Romero, Diego
A1 Ganfornina Álvarez, María Dolores
A1 Gutiérrez, Gabriel
A1 Marín, Antonio
K1 Lipocainas
K1 Calciproteinas
AB The Lipocalins are an ancient protein family whose expression is currently confirmed in bacteria, protoctists, plants,arthropods, and chordates. The evolution of this protein family has been assessed previously using amino acid sequencephylogenies. In this report we use an independent set of characters derived from the gene structure (exon-intronarrangement) to infer a new lipocalin phylogeny. We also present the novel gene structure of three insect lipocalins. Theposition and phase of introns are well preserved among lipocalin clades when mapped onto a protein sequence alignment,suggesting the homologous nature of these introns. Because of this homology, we use the intron position and phase of 23lipocalin genes to reconstruct a phylogeny by maximum parsimony and distance methods. These phylogenies are verysimilar to the phylogenies derived from protein sequence. This result is confirmed by congruence analysis, anda consensus tree shows the commonalities between the two source trees. Interestingly, the intron arrangement phylogenyshows that metazoan lipocalins have more introns than other eukaryotic lipocalins, and that intron gains have occurred inthe C-termini of chordate lipocalins. We also analyze the relationship of intron arrangement and protein tertiary structure,as well as the relationship of lipocalins with members of the proposed structural superfamily of calycins. Our congruenceanalysis validates the gene structure data as a source of phylogenetic information and helps to further refine ourhypothesis on the evolutionary history of lipocalins.
PB Society for Molecular Biology and Evolution
SN 0737-4038
YR 2003
FD 2003
LK http://uvadoc.uva.es/handle/10324/6102
UL http://uvadoc.uva.es/handle/10324/6102
LA eng
NO Molecular Biology and Evolution, 2003, vol. 20, n. 5, p. 775-783
NO Innovación Educativa
DS UVaDOC
RD 22-nov-2024