RT info:eu-repo/semantics/article
T1 Structure and biological properties of ribosome-inactivating proteins and lectins from elder (Sambucus nigra L.) leaves
A1 Iglesias Álvarez, María del Rosario
A1 Russo, Rosita
A1 Landi, Nicola
A1 Valletta, Mariangela
A1 Chambery, Angela
A1 Di Maro, Antimo
A1 Bolognesi, Andrea
A1 Ferreras Rodríguez, José Miguel
A1 Citores González, Lucía
K1 Antineoplastic agents
K1 Lectines
K1 Lectinas - Purificación
K1 Proteins - Synthesis
K1 Proteínas - Síntesis
K1 Ribosomes - Structure
K1 Trees - Therapeutic use
K1 Árboles -Uso terapéutico
K1 Medicinal plants
K1 Plantas medicinales
K1 Ribosome-inactivating protein
K1 Proteína inactivadora de ribosomas
K1 Sambucus nigra L.
K1 2302.27 Proteínas
K1 2306 Química Orgánica
AB Ribosome-inactivating proteins (RIPs) are a group of proteins with rRNA N-glycosylase activity that catalyze the removal of a specific adenine located in the sarcin–ricin loop of the large ribosomal RNA, which leads to the irreversible inhibition of protein synthesis and, consequently, cell death. The case of elderberry (Sambucus nigra L.) is unique, since more than 20 RIPs and related lectins have been isolated and characterized from the flowers, seeds, fruits, and bark of this plant. However, these kinds of proteins have never been isolated from elderberry leaves. In this work, we have purified RIPs and lectins from the leaves of this shrub, studying their main physicochemical characteristics, sequences, and biological properties. In elderberry leaves, we found one type 2 RIP and two related lectins that are specific for galactose, four type 2 RIPs that fail to agglutinate erythrocytes, and one type 1 RIP. Several of these proteins are homologous to others found elsewhere in the plant. The diversity of RIPs and lectins in the different elderberry tissues, and the different biological activities of these proteins, which have a high degree of homology with each other, constitute an excellent source of proteins that are of great interest in diagnostics, experimental therapy, and agriculture.
PB MDPI
SN 2072-6651
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/61196
UL https://uvadoc.uva.es/handle/10324/61196
LA eng
NO Toxins, 2022, Vol. 14, Nº. 9, 611
NO Producción Científica
DS UVaDOC
RD 07-ago-2024