RT info:eu-repo/semantics/article
T1 Analysis of bone histomorphometry in rat and guinea pig animal models subject to hypoxia
A1 Usategui Martín, Ricardo
A1 Real, Álvaro del
A1 Sainz-Aja Guerra, José A.
A1 Prieto Lloret, Jesús
A1 Olea Fraile, Elena
A1 Rocher Martín, María Asunción
A1 Rigual Bonastre, Ricardo Jaime
A1 Riancho Moral, José Antonio
A1 Pérez Castrillon, José Luis
K1 Hypoxia
K1 Hipoxia
K1 Biometry
K1 Biometría
K1 Bone remodeling
K1 Huesos - Regeneración
K1 Bone - Diseases
K1 Huesos - Enfermedades - Diagnóstico
K1 Obesity
K1 Obesidad
K1 Tomography
K1 Tomografía computada
K1 Animal Models
K1 Modelos animales
K1 Laboratory animals
K1 Animales de laboratorio
K1 Animal experimentation
K1 Experimentación animal
K1 2415 Biología Molecular
K1 3201.04 Patología Clínica
AB Hypoxia may be associated with alterations in bone remodeling, but the published results are contradictory. The aim of this study was to characterize the bone morphometry changes subject to hypoxia for a better understanding of the bone response to hypoxia and its possible clinical consequences on the bone metabolism. This study analyzed the bone morphometry parameters by micro-computed tomography (μCT) in rat and guinea pig normobaric hypoxia models. Adult male and female Wistar rats were exposed to chronic hypoxia for 7 and 15 days. Additionally, adult male guinea pigs were exposed to chronic hypoxia for 15 days. The results showed that rats exposed to chronic constant and intermittent hypoxic conditions had a worse trabecular and cortical bone health than control rats (under a normoxic condition). Rats under chronic constant hypoxia were associated with a more deteriorated cortical tibia thickness, trabecular femur and tibia bone volume over the total volume (BV/TV), tibia trabecular number (Tb.N), and trabecular femur and tibia bone mineral density (BMD). In the case of chronic intermittent hypoxia, rats subjected to intermittent hypoxia had a lower cortical femur tissue mineral density (TMD), lower trabecular tibia BV/TV, and lower trabecular thickness (Tb.Th) of the tibia and lower tibia Tb.N. The results also showed that obese rats under a hypoxic condition had worse values for the femur and tibia BV/TV, tibia trabecular separation (Tb.Sp), femur and tibia Tb.N, and BMD for the femur and tibia than normoweight rats under a hypoxic condition. In conclusion, hypoxia and obesity may modify bone remodeling, and thus bone microarchitecture, and they might lead to reductions in the bone strength and therefore increase the risk of fragility fracture.
PB MDPI
SN 1422-0067
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/61612
UL https://uvadoc.uva.es/handle/10324/61612
LA eng
NO International Journal of Molecular Sciences, 2022, Vol. 23, Nº. 21, 12742
NO Producción Científica
DS UVaDOC
RD 22-dic-2024