RT info:eu-repo/semantics/article
T1 Modulation of multiple gene clusters’ expression by the PAS-LuxR transcriptional regulator PteF
A1 Vicente, Cláudia
A1 Payero, Tamara
A1 Rodríguez García, Antonio
A1 Barreales, Eva
A1 Pedro, Antonio de
A1 Santos Beneit, Fernando
A1 Aparicio, Jesús F.
K1 Antifungal agents
K1 Genetic regulation
K1 Regulación genética
K1 Medical genetics
K1 Antibiotics
K1 Antibióticos
K1 Streptomyces
K1 Genética - Bacterias
K1 3303 Ingeniería y Tecnología Químicas
K1 3201.02 Genética Clínica
AB PAS-LuxR transcriptional regulators are conserved proteins governing polyene antifungal biosynthesis. PteF is the regulator of filipin biosynthesis from Streptomyces avermitilis. Its mutation drastically abates filipin, but also oligomycin production, a macrolide ATP-synthase inhibitor, and delays sporulation; thus, it has been considered a transcriptional activator. Transcriptomic analyses were performed in S. avermitilis ΔpteF and its parental strain. Both strains were grown in a YEME medium without sucrose, and the samples were taken at exponential and stationary growth phases. A total of 257 genes showed an altered expression in the mutant, most of them at the exponential growth phase. Surprisingly, despite PteF being considered an activator, most of the genes affected showed overexpression, thereby suggesting a negative modulation. The affected genes were related to various metabolic processes, including genetic information processing; DNA, energy, carbohydrate, and lipid metabolism; morphological differentiation; and transcriptional regulation, among others, but were particularly related to secondary metabolite biosynthesis. Notably, 10 secondary metabolite gene clusters out of the 38 encoded by the genome showed altered expression profiles in the mutant, suggesting a regulatory role for PteF that is wider than expected. The transcriptomic results were validated by quantitative reverse-transcription polymerase chain reaction. These findings provide important clues to understanding the intertwined regulatory machinery that modulates antibiotic biosynthesis in Streptomyces.
PB MDPI
SN 2079-6382
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/61984
UL https://uvadoc.uva.es/handle/10324/61984
LA eng
NO Antibiotics, 2022, Vol. 11, Nº. 8, 994
NO Producción Científica
DS UVaDOC
RD 24-nov-2024