RT info:eu-repo/semantics/article
T1 Lignin–chitosan nanocarriers for the delivery of bioactive natural products against wood-decay phytopathogens
A1 Sánchez Hernández, Eva
A1 Langa Lomba, Natalia
A1 González García, Vicente
A1 Casanova Gascón, José
A1 Martín Gil, Jesús
A1 Santiago Aliste, Alberto
A1 Torres Sánchez, Sergio
A1 Martín Ramos, Pablo
K1 Lignin
K1 Chitosan
K1 Nanotechnology
K1 Plant pathology
K1 Agriculture
K1 Grapevine trunk diseases
K1 Vid - Enfermedades y plagas
K1 Natural products
K1 Productos naturales
K1 Patologia vegetal
K1 Bioactive compounds
K1 Compuestos bioactivos
K1 Wood - Deterioration
K1 Madera  - Deterioro
K1 3102 Ingeniería Agrícola
K1 3103 Agronomía
K1 3103.09 Cultivos de Plantas
K1 3108 Fitopatología
AB The use of nanocarriers (NCs), i.e., nanomaterials capable of encapsulating drugs and releasing them selectively, is an emerging field in agriculture. In this study, the synthesis, characterization, and in vitro and in vivo testing of biodegradable NCs loaded with natural bioactive products was investigated for the control of certain phytopathogens responsible for wood degradation. In particular, NCs based on methacrylated lignin and chitosan oligomers, loaded with extracts from Rubia tinctorum, Silybum marianum, Equisetum arvense, and Urtica dioica, were first assayed in vitro against Neofusicoccum parvum, an aggressive fungus that causes cankers and diebacks in numerous woody hosts around the world. The in vitro antimicrobial activity of the most effective treatment was further explored against another fungal pathogen and two bacteria related to trunk diseases: Diplodia seriata, Xylophilus ampelinus, and Pseudomonas syringae pv. syringae, respectively. Subsequently, it was evaluated in field conditions, in which it was applied by endotherapy for the control of grapevine trunk diseases. In the in vitro mycelial growth inhibition tests, the NCs loaded with R. tinctorum resulted in EC90 concentrations of 65.8 and 91.0 μg·mL−1 against N. parvum and D. seriata, respectively. Concerning their antibacterial activity, a minimum inhibitory concentration of 37.5 μg·mL−1 was obtained for this treatment against both phytopathogens. Upon application via endotherapy on 20-year-old grapevines with clear esca and Botryosphaeria decay symptoms, no phytotoxicity effects were observed (according to SPAD and chlorophyll fluorescence measurements) and the sugar content of the grape juice was not affected either. Nonetheless, the treatment led to a noticeable decrease in foliar symptoms as well as a higher yield in the treated arms as compared to the control arms (3177 vs. 1932 g/arm), suggestive of high efficacy. Given the advantages in terms of controlled release and antimicrobial product savings, these biodegradable NCs loaded with natural extracts may deserve further research in large-scale field tests.
PB MDPI
SN 2073-4395
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/62164
UL https://uvadoc.uva.es/handle/10324/62164
LA eng
NO Agronomy, 2022, Vol. 12, Nº. 2, 461
NO Producción Científica
DS UVaDOC
RD 02-dic-2024