RT info:eu-repo/semantics/article T1 Biodegradable solvents: A promising tool to recover proteins from microalgae A1 Moldes Plaza, David A1 Rojo de Benito, Elena María A1 Bolado Rodríguez, Silvia A1 García Encina, Pedro Antonio A1 Comesaña Gándara, Bibiana K1 Solvents K1 Disolventes K1 Biodegradable K1 Cell disruption K1 Deep eutectic solvents K1 Extraction (Chemistry) K1 Extracción (Química) K1 Ionic liquids K1 Líquidos K1 Microalgae K1 Biorefinery K1 Microalgae - Biotechnology K1 Biomass energy K1 Renewable energy resources K1 Recursos energéticos renovables K1 Plant biochemistry K1 Materials science K1 Proteins K1 Technological innovations K1 Innovaciones tecnológicas K1 3303 Ingeniería y Tecnología Químicas K1 3308 Ingeniería y Tecnología del Medio Ambiente K1 2510.91 Recursos Renovables AB The world will face a significant protein demand in the next few decades, and due to the environmental concerns linked to animal protein, new sustainable protein sources must be found. In this regard, microalgae stand as an outstanding high-quality protein source. However, different steps are needed to separate the proteins from the microalgae biomass and other biocompounds. The protein recovery from the disrupted biomass is usually the bottleneck of the process, and it typically employs organic solvents or harsh conditions, which are both detrimental to protein stability and planet health. Different techniques and methods are applied for protein recovery from various matrices, such as precipitation, filtration, chromatography, electrophoresis, and solvent extraction. Those methods will be reviewed in this work, discussing their advantages, drawbacks, and applicability to the microalgae biorefinery process. Special attention will be paid to solvent extraction performed with ionic liquids (ILs) and deep eutectic solvents (DESs), which stand as promising solvents to perform efficient protein separations with reduced environmental costs compared to classical alternatives. Finally, several solvent recovery options will be analyzed to reuse the solvent employed and isolate the proteins from the solvent phase. PB MDPI SN 2076-3417 YR 2022 FD 2022 LK https://uvadoc.uva.es/handle/10324/62219 UL https://uvadoc.uva.es/handle/10324/62219 LA eng NO Applied Sciences, 2022, Vol. 12, Nº. 5, 2391 NO Producción Científica DS UVaDOC RD 24-nov-2024