RT info:eu-repo/semantics/article T1 Ectoines production from biogas in pilot bubble column bioreactors and their subsequent extraction via bio-milking A1 Rodero Raya, María del Rosario A1 Carmona Martínez, Alessandro A. A1 Martínez Fraile, Cristina A1 Herrero Lobo, Raquel A1 Rodríguez Rodríguez, Elisa A1 García Encina, Pedro Antonio A1 Peña Miranda, María del Mar Agripina A1 Muñoz Torre, Raúl K1 Medio ambiente, Ingeniería del K1 Renewable and Green Energy K1 Ectoine K1 Biogas valorization K1 Methanotrophs K1 Ectoína K1 Valorización de biogás K1 Metanotrofos K1 3308 Ingeniería y Tecnología del Medio Ambiente AB Despite the potential of biogas from waste/wastewater treatment as a renewable energy source, the presence of pollutants and the rapid decrease in the levelized cost of solar and wind power constrain the use of biogas for energy generation. Biogas conversion into ectoine, one of the most valuable bioproducts (1000 €/kg), constitutes a new strategy to promote a competitive biogas market. The potential for a stand-alone 20 L bubble column bioreactor operating at 6% NaCl and two 10 L interconnected bioreactors (at 0 and 6% NaCl, respectively) for ectoine production from biogas was comparatively assessed. The stand-alone reactor supported the best process performance due to its highest robustness and efficiency for ectoine accumulation (20–52 mgectoine/gVSS) and CH4 degradation (up to 84%). The increase in N availability and internal gas recirculation did not enhance ectoine synthesis. However, a 2-fold increase in the internal gas recirculation resulted in an approximately 1.3-fold increase in CH4 removal efficiency. Finally, the recovery of ectoine through bacterial bio-milking resulted in efficiencies of >70% without any negative impact of methanotrophic cell recycling to the bioreactors on CH4 biodegradation or ectoine synthesis. PB Elsevier SN 0043-1354 YR 2023 FD 2023 LK https://uvadoc.uva.es/handle/10324/62510 UL https://uvadoc.uva.es/handle/10324/62510 LA eng NO Water Research, 2023, vol. 245, 120665 NO Producción Científica DS UVaDOC RD 23-dic-2024