RT info:eu-repo/semantics/article T1 Detection of SARS-CoV-2 in the indoor and outdoor areas of urban public transport systems of three major cities of Portugal in 2021 A1 Gomes da Silva, Priscilla A1 Gonçalves, José A1 Nascimento, Maria São José A1 Sousa, Sofia I. V. A1 Mesquita, João K1 SARS-CoV-2 K1 COVID-19 K1 Airborne transmission K1 Environmental pollution K1 Contaminación ambiental K1 Indoor air quality K1 Calidad ambiental interior K1 Indoor air pollution K1 Outdoor air pollution K1 Contaminación del aire K1 Air sampling K1 Air - Pollution K1 Aire - Contaminación K1 Public transport K1 Transporte público K1 Medical care -- Portugal K1 Public Health K1 2509.02 Contaminación Atmosférica K1 3308 Ingeniería y Tecnología del Medio Ambiente K1 3212 Salud Publica AB Airborne transmission is mainly associated with poorly ventilated and crowded indoor environments where people stay for long periods of time. As such, public transport is often perceived as having a high risk for the transmission of SARS-CoV-2. Considering that data on the detection of SARS-CoV-2 in public transport systems are scarce, we performed air sampling for SARS-CoV-2 in indoor and outdoor spaces of public transport systems in Portugal. Air (n = 31) and surface (n = 70) samples were collected using a Coriolis® Compact microbial air sampler and sterile flocked plastic swabs, respectively. Samples were extracted and analyzed through RT-qPCR. Only two air samples from an outdoor and a partially open space were found to be positive for SARS-CoV-2 RNA. No positive surface samples were detected. These results indicate that the viral concentration in ambient air in public transport systems is linked to the number of people present in that environment and whether they are wearing properly fitting masks. Considering the current lifting of COVID-19 restrictions around the world, it is essential that people continue to wear masks in both indoor and outdoor environments, especially in crowded spaces. More studies on this topic are needed to fully elucidate the real risk of infection in outdoor spaces. PB MDPI SN 1660-4601 YR 2022 FD 2022 LK https://uvadoc.uva.es/handle/10324/62727 UL https://uvadoc.uva.es/handle/10324/62727 LA eng NO International Journal of Environmental Research and Public Health, 2022, Vol. 19, Nº. 10, 5955 NO Producción Científica DS UVaDOC RD 12-sep-2024