RT info:eu-repo/semantics/article
T1 Differential expression and localization of transient receptor potential vanilloid 1 in rabbit and human eyes
A1 Martínez García, María del Carmen
A1 Martínez Valcárcel, Tamara
A1 Pañeda Vázquez-Prada, Covadonga
A1 Gallego Muñoz, Patricia
A1 Jimenez, Ana I.
A1 Merayo Lloves, Jesús
K1 Cornea - Enfermedades - Tratamiento
AB Summary. Introduction: The superfamily of transientreceptor potential (TRP) cation channels is involved innociception. Members of this family, such as thevanilloid receptor type 1 (TRPV1) channel, are activatedby a wide range of stimuli including heat (>43°C), lowpH (<6.5), hypoxia, and hypertonicity. Here we reportTRPV1 expression in rabbit and human eyes.Material and methods: We analyzed the expressionof TRPV1 mRNA by quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR) and protein byimmunohistochemistry in eyes of New Zealand Whiterabbits and humans.Results: In rabbit and human eyes, TRPV1 proteinwas present in all layers of the corneal epithelium, butonly in the basal layer of the conjunctiva. It was also inthe ciliary and lens epithelia of both species as well as inthe secretory cells of the rabbit lacrimal gland. Theretinal pigment epithelium was positive for this proteinin both species. TRPV1 was also present in rabbit Müllercells, where it had a similar pattern of expression tovimentin intermediate filaments. Analysis by qRT-PCRshowed that TRPV1 mRNA was found in all of thestructures where the protein was present. The highestlevel was in the lens and the lowest in the retina.Conclusion: TRPV1 is expressed in cells that areparticularly active in Ca2+ exchange as well as in cellswith significant water transport activity. Because TRPV1is a Ca2+ channel, it probably functions in the regulationof both water and Ca2+ movements in ocular tissues.
PB Universidad de Murcia
SN 0213-3911
YR 2013
FD 2013
LK http://uvadoc.uva.es/handle/10324/6273
UL http://uvadoc.uva.es/handle/10324/6273
LA eng
NO Histology and Histopatology, 2013, vol. 28, p. 1507-1516
NO Producción Científica
DS UVaDOC
RD 24-nov-2024