RT info:eu-repo/semantics/article
T1 Oleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis
A1 Córdova, Claudia
A1 Gutiérrez, Beatriz
A1 Martínez García, María del Carmen
A1 Martín, Rubén
A1 Gallego Muñoz, Patricia
A1 Hernández Garrido, Marita
A1 Nieto Salinas, María de la Paz
K1 Conjuntivitis alérgica - Tratamiento
AB Pollen is the most common aeroallergen to cause seasonal conjunctivitis. The result of allergen exposure is a strong Th2-mediated response along with conjunctival mast cell degranulation and eosinophilic infiltration. Oleanolic acid (OA) isnatural a triterpene that displays strong anti-inflammatory and immunomodulatory properties being an active anti-allergicmolecule on hypersensitivity reaction models. However, its effect on inflammatory ocular disorders including conjunctivits,has not yet been addressed. Hence, using a Ragweed pollen (RWP)-specific allergic conjunctivitis (EAC) mouse model westudy here whether OA could modify responses associated to allergic processes. We found that OA treatment restrictedmast cell degranulation and infiltration of eosinophils in conjunctival tissue and decreased allergen-specific Igs levels in EACmice. Th2-type cytokines, secreted phospholipase A2 type-IIA (sPLA2-IIA), and chemokines levels were also significantlydiminished in the conjunctiva and serum of OA-treated EAC mice. Moreover, OA treatment also suppressed RWP-specific Tcellproliferation. In vitro studies, on relevant cells of the allergic process, revealed that OA reduced the proliferative andmigratory response, as well as the synthesis of proinflammatory mediators on EoL-1 eosinophils and RBL-2H3 mast cellsexposed to allergic and/or crucial inflammatory stimuli such as RWP, sPLA2-IIA or eotaxin. Taken together, these findingsdemonstrate the beneficial activity of OA in ocular allergic processes and may provide a new intervention strategy andpotential therapy for allergic diseases.
PB Macau University of Science and Technology
SN 1932-6203
YR 2014
FD 2014
LK http://uvadoc.uva.es/handle/10324/6287
UL http://uvadoc.uva.es/handle/10324/6287
LA eng
NO PLos ONE, 2014, vol. 9, n. 4. p. 1-11
NO Producción Científica
DS UVaDOC
RD 05-nov-2024