RT info:eu-repo/semantics/article
T1 Deciphering molecular determinants underlying Penicillium digitatum’s response to biological and chemical antifungal agents by tandem mass tag (TMT)-based high-resolution LC-MS/MS
A1 Citores González, Lucía
A1 Valletta, Mariangela
A1 Singh, Vikram Pratap
A1 Pedone, Paolo Vincenzo
A1 Iglesias Álvarez, María del Rosario
A1 Ferreras Rodríguez, José Miguel
A1 Chambery, Angela
A1 Russo, Rosita
K1 Penicillium digitatum
K1 Proteomics
K1 Proteómica
K1 Ribosome inactivating protein
K1 Ribosomes - Structure
K1 Proteins - Synthesis
K1 Ribotoxin
K1 Fungi
K1 Molds (Fungi)
K1 Citrus
K1 Cítricos
K1 Plant pathology
K1 Enfermedades de las plantas
K1 Fungal diseases of plants
K1 Biochemistry
K1 Molecular biology
K1 2302.27 Proteínas
K1 3108.05 Hongos
K1 2414.09 Mohos
K1 2302 Bioquímica
K1 2302.21 Biología Molecular
AB Penicillium digitatum is a widespread pathogen responsible for the postharvest decay of citrus, one of the most economically important crops worldwide. Currently, chemical fungicides are still the main strategy to control the green mould disease caused by the fungus. However, the increasing selection and proliferation of fungicide-resistant strains require more efforts to explore new alternatives acting via new or unexplored mechanisms for postharvest disease management. To date, several non-chemical compounds have been investigated for the control of fungal pathogens. In this scenario, understanding the molecular determinants underlying P. digitatum’s response to biological and chemical antifungals may help in the development of safer and more effective non-chemical control methods. In this work, a proteomic approach based on isobaric labelling and a nanoLC tandem mass spectrometry approach was used to investigate molecular changes associated with P. digitatum’s response to treatments with α-sarcin and beetin 27 (BE27), two proteins endowed with antifungal activity. The outcomes of treatments with these biological agents were then compared with those triggered by the commonly used chemical fungicide thiabendazole (TBZ). Our results showed that differentially expressed proteins mainly include cell wall-degrading enzymes, proteins involved in stress response, antioxidant and detoxification mechanisms and metabolic processes such as thiamine biosynthesis. Interestingly, specific modulations in response to protein toxins treatments were observed for a subset of proteins. Deciphering the inhibitory mechanisms of biofungicides and chemical compounds, together with understanding their effects on the fungal physiology, will provide a new direction for improving the efficacy of novel antifungal formulations and developing new control strategies.
PB MDPI
SN 1422-0067
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/62924
UL https://uvadoc.uva.es/handle/10324/62924
LA eng
NO International Journal of Molecular Sciences, 2022, Vol. 23, Nº. 2, 680
NO Producción Científica
DS UVaDOC
RD 26-jun-2024