RT info:eu-repo/semantics/article
T1 Potential ophthalmological application of extracts obtained from tuna vitreous humor using lactic acid-based deep eutectic systems
A1 Abdallah, Maha M.
A1 Leonardo, Inês C.
A1 Krstić, Luna
A1 Enriquez De Salamanca Aladro, Amalia
A1 Diebold Luque, María Yolanda
A1 González García, María Jesús
A1 Gaspar, Frédéric Bustos
A1 Matias, Ana A.
A1 Bronze, Maria Rosario
A1 Fernández, Naiara
K1 Food waste
K1 Residuos alimentarios
K1 Atunes
K1 Hyaluronic acid
K1 Acido hialurónico - Uso terapéutico
K1 Deep eutectic solvents
K1 Disolventes
K1 Ophthalmology
K1 Ocular pharmacology
K1 Antioxidants
K1 Antioxidantes
K1 Anti-inflammatory
K1 Antiinflamatorios
K1 Antimicrobial
K1 Microbiology
K1 Eye - Diseases
K1 Ojos - Enfermedades
K1 Dry eye syndromes
K1 Síndrome de ojo seco
K1 3201.09 Oftalmología
K1 3209 Farmacología
K1 2414 Microbiología
AB A green technique was developed to extract hyaluronic acid (HA) from tuna vitreous humor (TVH) for its potential application in managing dry eye disease. Deep eutectic solvents (DES) were used to extract HA and were synthesized using natural compounds (lactic acid, fructose, and urea). The DES, the soluble fraction of TVH in DES (SF), and the precipitated extracts (PE) were evaluated for their potential use in dry eye disease treatment. In vitro experiments on human corneal epithelial cell lines and the effect on dry eye-associated microorganisms were performed. The influence of the samples on the HCE viability, their intracellular reactive oxygen species (ROS) scavenging capacity, inflammatory response, and antimicrobial properties were studied. According to the results, all samples displayed an antioxidant effect, which was significantly higher for PE in comparison to SF. Most of the tested samples did not induce an inflammatory response in cells, which confirmed the safety in ophthalmic formulations. In addition, the DES and SF proved to be efficient against the studied bacterial strains, while PE did not show an antimicrobial effect. Hence, both DES and SF at defined concentrations could be used as potential compounds in dry eye disease management.
PB MDPI
SN 2304-8158
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/63112
UL https://uvadoc.uva.es/handle/10324/63112
LA eng
NO Foods, 2022, Vol. 11, Nº. 3, 342
NO Producción Científica
DS UVaDOC
RD 24-nov-2024