RT info:eu-repo/semantics/article
T1 Screening enzymes that can depolymerize commercial biodegradable polymers: Heterologous expression of Fusarium solani cutinase in Escherichia coli
A1 Santos Beneit, Fernando
A1 Chen, Le Min
A1 Bordel Velasco, Sergio
A1 Frutos de la Flor, Raquel
A1 García Depraect, Octavio
A1 Lebrero Fernández, Raquel
A1 Rodriguez Vega, Sara
A1 Muñoz Torre, Raúl
A1 Börner, Rosa Aragão
A1 Börner, Tim
K1 Biodegradable plastics
K1 Polymeric composites
K1 Depolymerization
K1 Polymers
K1 Polymerization
K1 Polimeros y polimerizacion
K1 Plastics
K1 Materias plásticas
K1 Sustainable development
K1 Desarrollo sostenible
K1 Renewable natural resources
K1 Recursos naturales renovables
K1 Enzymes
K1 Enzimas
K1 Microbiology
K1 Environment
K1 2206.10 Polímeros
K1 2302.09 Enzimología
K1 2414 Microbiología
K1 3308 Ingeniería y Tecnología del Medio Ambiente
AB In recent years, a number of microbial enzymes capable of degrading plastics have been identified. Biocatalytic depolymerization mediated by enzymes has emerged as a potentially more efficient and environmentally friendly alternative to the currently employed methods for plastic treatment and recycling. However, the functional and systematic study of depolymerase enzymes with respect to the degradation of a series of plastic polymers in a single work has not been widely addressed at present. In this study, the ability of a set of enzymes (esterase, arylesterase and cutinase) to degrade commercial biodegradable polymers (PBS, PBAT, PHB, PHBH, PHBV, PCL, PLA and PLA/PCL) and the effect of pre-treatment methods on their degradation rate was assessed. The degradation products were identified and quantified by HPLC and LC-HRMS analysis. Out of the three enzymes, Fusarium solani cutinase (FsCut) showed the highest activity on grinded PBAT, PBS and PCL after 7 days of incubation. FsCut was engineered and heterologous expressed in Escherichia coli, which conferred the bacterium the capability of degrading solid discs of PBAT and to grow in PBS as the sole carbon source of the medium.
PB MDPI
SN 2076-2607
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/63479
UL https://uvadoc.uva.es/handle/10324/63479
LA eng
NO Microorganisms, 2023, Vol. 11, Nº. 2, 328
NO Producción Científica
DS UVaDOC
RD 27-jun-2024