RT info:eu-repo/semantics/article
T1 GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in organelles
A1 Rodríguez García, Arancha
A1 Rojo Ruiz, Jonathan
A1 Navas Navarro, Paloma
A1 Aulestia Araya, Francisco Javier
A1 Gallego Sandin, Sonia
A1 García-Sancho Martín, Francisco Javier
A1 Alonso Alonso, María Teresa
K1 Calcio
K1 Radiología
AB Genetically encoded calcium indicators allow monitoring subcellularCa2+ signals inside organelles. Most genetically encoded calciumindicators are fusions of endogenous calcium-binding proteinswhose functionality in vivo may be perturbed by competition withcellular partners.We describe here a novel family of fluorescent Ca2+sensors based on the fusion of two Aequorea victoria proteins, GFPand apo-aequorin (GAP). GAP exhibited a unique combinationof features: dual-excitation ratiometric imaging, high dynamicrange, good signal-to-noise ratio, insensitivity to pH and Mg2+,tunable Ca2+ affinity, uncomplicated calibration, and targetabilityto five distinct organelles. Moreover, transgenic mice for endoplasmicreticulum-targeted GAP exhibited a robust long-termexpression that correlated well with its reproducible performance invarious neural tissues. This biosensor fills a gap in the actual repertoireof Ca2+ indicators for organelles and becomes a valuable toolfor in vivo Ca2+ imaging applications.
PB National Academy of Sciences
SN 1091-6490
YR 2014
FD 2014
LK http://uvadoc.uva.es/handle/10324/6350
UL http://uvadoc.uva.es/handle/10324/6350
LA eng
NO Proceedings of the National Academy of Sciences, Febrero 2014, vol. 111, n. 7, p. 2584-2589
NO Producción Científica
DS UVaDOC
RD 23-nov-2024