RT info:eu-repo/semantics/acceptedVersion
T1 Single-Cell Redox Imaging Demonstrates a Distinctive Response of Dopaminergic Neurons to Oxidative Insults
A1 Horowitz, Maxx P.
A1 Milanese, Chiara
A1 Di Maio, Roberto
A1 Hu, Xiaoping
A1 Montero, Laura M.
A1 Sanders, Laurie H.
A1 Tapias, Victor
A1 Sepe, Sara
A1 van Cappellen, Wiggert A.
A1 Burton, Edward A.
A1 Greenamyre, John Timothy
A1 Mastroberardino, Pier G.
AB Aims: The study of the intracellular oxido-reductive (redox) state is of extreme relevance to the dopamine (DA) neurons of the substantia nigra pars compacta. These cells possess a distinct physiology intrinsically associated with elevated reactive oxygen species production, and they selectively degenerate in Parkinson's disease under oxidative stress conditions. To test the hypothesis that these cells display a unique redox response to mild, physiologically relevant oxidative insults when compared with other neuronal populations, we sought to develop a novel method for quantitatively assessing mild variations in intracellular redox state.Results: We have developed a new imaging strategy to study redox variations in single cells, which is sensitive enough to detect changes within the physiological range. We studied DA neurons' physiological redox response in biological systems of increasing complexity--from primary cultures to zebrafish larvae, to mammalian brains-and identified a redox response that is distinctive for substantia nigra pars compacta DA neurons. We studied simultaneously, and in the same cells, redox state and signaling activation and found that these phenomena are synchronized.Innovation: The redox histochemistry method we have developed allows for sensitive quantification of intracellular redox state in situ. As this method is compatible with traditional immunohistochemical techniques, it can be applied to diverse settings to investigate, in theory, any cell type of interest.Conclusion: Although the technique we have developed is of general interest, these findings provide insights into the biology of DA neurons in health and disease and may have implications for therapeutic intervention.
SN 1523-0864
YR 2011
FD 2011
LK https://uvadoc.uva.es/handle/10324/63876
UL https://uvadoc.uva.es/handle/10324/63876
LA eng
NO Antioxidants & Redox Signaling, Agosto 2011, vol. 15, n. 4, p. 855-871
NO Producción Científica
DS UVaDOC
RD 16-jun-2024