RT info:eu-repo/semantics/article
T1 Lipid Droplet Biogenesis Induced by Stress Involves Triacylglycerol Synthesis That Depends on Group VIA Phospholipase A2
A1 Gubern, Albert
A1 Barceló-Torns, Miquel
A1 Casas, Javier
A1 Barneda, David
A1 Masgrau, Roser
A1 Picatoste, Fernando
A1 Balsinde, Jesús
A1 Balboa, María A.
A1 Claro, Enrique
AB This work investigates the metabolic origin of triacylglycerol (TAG) formed during lipid droplet (LD) biogenesis induced by stress. Cytotoxic inhibitors of fatty acid synthase induced TAG synthesis and LD biogenesis in CHO-K1 cells, in the absence of external sources of fatty acids. TAG synthesis was required for LD biogenesis and was sensitive to inhibition and down-regulation of the expression of group VIA phospholipase A(2) (iPLA(2)-VIA). Induction of stress with acidic pH, C(2)-ceramide, tunicamycin, or deprivation of glucose also stimulated TAG synthesis and LD formation in a manner dependent on iPLA(2)-VIA. Overexpression of the enzyme enhanced TAG synthesis from endogenous fatty acids and LD occurrence. During stress, LD biogenesis but not TAG synthesis required phosphorylation and activation of group IVA PLA(2) (cPLA(2)alpha). The results demonstrate that iPLA(2)-VIA provides fatty acids for TAG synthesis while cPLA(2)alpha allows LD biogenesis. LD biogenesis during stress may be a survival strategy, recycling structural phospholipids into energy-generating substrates.
SN 0021-9258
YR 2009
FD 2009
LK https://uvadoc.uva.es/handle/10324/64295
UL https://uvadoc.uva.es/handle/10324/64295
LA eng
NO The Journal of biological chemistry 284, 5697–5708 (2009).
NO Producción Científica
DS UVaDOC
RD 12-sep-2024