RT info:eu-repo/semantics/article
T1 Stem Cell Therapy for Corneal Epithelium Regeneration following Good Manufacturing and Clinical Procedures
A1 Ramírez, Beatriz E.
A1 Sánchez García, Ana María de los Ángeles
A1 Herreras Cantalapiedra, José María
A1 Fernández Martínez, Itziar
A1 García-Sancho Martín, Francisco Javier
A1 Nieto Miguel, Teresa
A1 Calonge, Margarita
AB Objective. To evaluate outcomes of cultivated limbal epithelial transplantation (CLET) for management of ocular surface failure due to limbal stem cell deficiency (LSCD).Design. Prospective, noncomparative, interventional case series and extensive comparison with recent similar studies.Participants. Twenty eyes with LSCD underwent CLET (11 autologous; 9 allogeneic) and were followed up for 3 years. Etiologies were divided into 3 prognostic categories: Group 1, chemical injuries (7 eyes); Group 2, immune-based inflammation (4 eyes); and Group 3, noninflammatory diseases (9 eyes).Intervention. Autologous and allogeneic limbal epithelial cells were cultivated on amniotic membranes and transplanted. Evaluations were based on clinical parameters, survival analysis, and in vivo confocal microscopy (IVCM). European Union Tissues/Cells Directive and good manufacturing procedures were followed.Main Outcome Measures. Improved clinical parameters, absence of epithelial defects, and improved central corneal epithelial phenotype.Results. Success rate was 80% at 1-2 years and 75% at 3 years. Autografts and allografts had similar survival. Success rate was significantly lower in prognostic Group 1 (42.9%) than in Groups 2-3 (100% each). All clinical parameters improved substantially. By IVCM, 80% of cases improved in epithelial status.Conclusions. CLET improved corneal epithelium quality, with subsequent improvement in symptoms, quality of life, and vision. These results confirm that CLET is a valid therapy for ocular surface failure.
PB Hindawi Publishing Corporation
SN 2314-6133
YR 2015
FD 2015
LK https://uvadoc.uva.es/handle/10324/64507
UL https://uvadoc.uva.es/handle/10324/64507
LA eng
NO BioMed Research Intternational. 2015;2015:408495. p. 1-19
NO Producción Científica
DS UVaDOC
RD 17-jul-2024