RT info:eu-repo/semantics/article
T1 Chitosan–gelatin biopolymers as carrier substrata for limbal epithelial stem cells
A1 De La Mata Sampedro, Ana
A1 Nieto Miguel, Teresa
A1 López Paniagua, Marina
A1 Galindo de la Rosa, Sara
A1 Aguilar, María Rosa
A1 García-Fernández, Luis
A1 Gonzalo, Sandra
A1 Vázquez, Blanca
A1 San Román del Barrio, Julio
A1 Corrales, Rosa María
A1 Calonge, Margarita
A1 De La Mata Sampedro, Ana
A1 Nieto-Miguel, Teresa
A1 López-Paniagua, Marina
A1 Galindo, Sara
A1 Román, Julio San
A1 Calonge, Margarita
AB The aim of this work was to evaluate semi-synthetic biopolymers based on chitosan (CH) and gelatin (G) as potential in vitro carrier substrata for human limbal epithelial cells (hLECs). To that end, human corneal epithelial cells (HCE) were cultured onto different CH–G membranes. None of the polymers were cytotoxic and cell proliferation was higher when CH was functionalized with G. Expression levels of corneal epithelial markers (K3, K12, E-caherin, desmoplakin, and zonula occludens (ZO)-1) were better maintained in HCE cells grown on CH–G 20:80 membranes than other proportions. Consequently, CH–G 20:80 was chosen for the subsequent expansion of hLECs. Cells derived from limbal explants were successfully expanded on CH–G 20:80 membranes using a culture medium lacking components of non-human animal origin. The expression levels found for corneal (K3 and K12) and limbal epithelial stem cells (K15) specific markers were similar to or higher than those found in limbal cells grown onto the control substratum. Our results demonstrate that CH–G 20:80 membranes are suitable for the expansion and maintenance of stem cells derived from the limbal niche. These results strongly support the use of polymers as alternative substrata for the transplantation of cultivated limbal cells onto the ocular surface.
PB SPRINGER
SN 0957-4530
YR 2013
FD 2013
LK https://uvadoc.uva.es/handle/10324/64508
UL https://uvadoc.uva.es/handle/10324/64508
LA eng
NO Journal of Materials Science: Materials in Medicine, 24, 2819–2829 (2013)
NO Producción Científica
DS UVaDOC
RD 07-ago-2024