RT info:eu-repo/semantics/article T1 Role of Oxidative Stress and Lipid Peroxidation in the Pathophysiology of NAFLD A1 Martín Fernández, Marta A1 Arroyo, Víctor A1 Carnicero, Carmen A1 Sigüenza, Rebeca A1 Busta, Reyes A1 Mora, Natalia A1 Antolín, Beatriz A1 Tamayo Gómez, Eduardo A1 Aspichueta, Patricia A1 Carnicero-Frutos, Irene A1 Gonzalo Benito, Hugo A1 Aller de la Fuente, Rocío AB Non-alcoholic fatty liver disease (NAFLD) is characterised by an excess of hepatic fat that can progress to steatohepatitis, fibrosis, cirrhosis and hepatocarcinoma. The imbalance between lipid uptake/lipogenesis and lipid oxidation/secretion in the liver is a major feature of NAFLD. Given the lack of a non-invasive and reliable methods for the diagnosis of non-alcoholic steatohepatitis (NASH), it is important to find serum markers that are capable of discriminating or defining patients with this stage of NASH. Blood samples were obtained from 152 Caucasian subjects with biopsy-proven NAFLD due to persistently elevated liver enzyme levels. Metabolites representative of oxidative stress were assessed. The findings derived from this work revealed that NAFLD patients with a NASH score of ≥ 4 showed significantly higher levels of lipid peroxidation (LPO). Indeed, LPO levels above the optimal operating point (OOP) of 315.39 μM are an independent risk factor for presenting a NASH score of ≥ 4 (OR: 4.71; 95% CI: 1.68−13.19; p = 0.003). The area under the curve (AUC = 0.81, 95% CI = 0.73−0.89, p < 0.001) shows a good discrimination ability of the model. Therefore, understanding the molecular mechanisms underlying the basal inflammation present in these patients is postulated as a possible source of biomarkers and therapeutic targets in NASH. PB MDPI YR 2022 FD 2022 LK https://uvadoc.uva.es/handle/10324/64525 UL https://uvadoc.uva.es/handle/10324/64525 LA spa NO Antioxidants (Basel). 2022 Nov 10;11(11):2217 DS UVaDOC RD 08-oct-2024