RT info:eu-repo/semantics/article
T1 Gene expression changes in conjunctival cells associated with contact lens wear and discomfort
A1 Calderón García, Andrés Ángel
A1 Valencia Nieto, Laura
A1 Valencia Sandonis, Cristina
A1 López de la Rosa, Alberto
A1 Blanco Vázquez, Marta
A1 Fernández Martínez, Itziar
A1 García Vázquez, Carmen
A1 Arroyo del Arroyo, Cristina
A1 González García, María Jesús
A1 Enriquez De Salamanca Aladro, Amalia
K1 Lentes de contacto
K1 Oftalmología
K1 Conjunctival cells
K1 Contact lens
K1 Discomfort
K1 Células conjuntivales
K1 Lentes de contacto
K1 Malestar
K1 3201.09 Oftalmología
AB This study aimed to analyze the differences in the expression of pain-related genes in conjunctival epithelial cells among symptomatic contact lens (CL) wearers (SCLWs), asymptomatic CL wearers (ACLWs), and non-CL wearers (non-CLWs).For this study, 60 participants (20 non-CLWs, 40 CLWs) were enrolled. The CLW group comprised 20 ACLWs and 20 SCLWs according to the Contact Lens Dry Eye Questionnaire short form©. Conjunctival cells were collected using impression cytology, and RNA was isolated and used to determine the expression levels of 85 human genes involved in neuropathic and inflammatory pain. The effects of CL wear and discomfort were evaluated using mixed-effects ANOVA with partially nested fixed-effects model. Gene set enrichment analysis was performed to assign biological meaning to sets of differentially expressed genesSix genes (CD200, EDN1, GRIN1, PTGS1, P2RX7, and TNF) were significantly upregulated in CLWs compared to non-CLWs. Eleven genes (ADORA1, BDKRB1, CACNA1B, DBH, GRIN1, GRM1, HTR1A, PDYN, PTGS1, P2RX3, and TNF) were downregulated in SCLWs compared to ACLWs. These genes were mainly related to pain, synaptic transmission and signaling, ion transport, calcium transport and concentration, and cell-cell signaling.CL wear modified the expression of pain- and inflammation-related genes in conjunctival epithelial cells. These changes may be in part, along with other mechanisms, responsible for CL discomfort in SCLWs.
PB Elsevier
SN 1542-0124
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/64577
UL https://uvadoc.uva.es/handle/10324/64577
LA eng
NO The Ocular Surface, 2024, vol. 31, p. 31-42
NO Producción Científica
DS UVaDOC
RD 17-jul-2024