RT info:eu-repo/semantics/article
T1 Combined quantification of procalcitonin and HLA-DR improves sepsis detection in surgical patients
A1 Almansa, Raquel
A1 Martín, Silvia
A1 Martin-Fernandez, Marta
A1 Heredia-Rodríguez, María
A1 Gómez-Sánchez, Esther
A1 Aragón, Marta
A1 Andrés, Cristina
A1 Calvo, Dolores
A1 Rico-Feijoo, Jesus
A1 Esteban-Velasco, Maria Carmen
A1 Vaquero-Roncero, Luis Mario
A1 Ortega, Alicia
A1 Gómez-Pesquera, Estefania
A1 Lorenzo-López, Mario
A1 de Cenarruzabeitia, Iñigo López
A1 Benavides, Diana
A1 López-Sanchez, Jaime
A1 Doncel, Cristina
A1 González-Sanchez, Carmen
A1 Zarca, Esther
A1 Ríos-Llorente, Alberto
A1 Diaz, Agustín
A1 Sanchez-Barrado, Elisa
A1 de Heredia, Juan Beltran
A1 Calvo-Vecino, Jose Maria
A1 Muñoz-Bellvís, Luis
A1 Gomez-Herreras, Jose Ignacio
A1 Aldecoa, César
A1 Tamayo, Eduardo
A1 Bermejo-Martin, Jesus F.
AB Early recognition of sepsis is a key factor to improve survival to this disease in surgical patients, since it allows prompt control of the infectious source. Combining pro-inflammatory and immunosupression biomarkers could represent a good strategy to improve sepsis detection. Here we evaluated the combination of procalcitonin (PCT) with gene expression levels of HLA-DRA to detect sepsis in a cohort of 154 surgical patients (101 with sepsis and 53 with no infection). HLA-DRA expression was quantified using droplet digital PCR, a next-generation PCR technology. Area under the receiver operating curve analysis (AUROC) showed that the PCT/HLA-DRA ratio outperformed PCT to detect sepsis (AUROC [CI95%], p): PCT: 0.80 [0.73-0.88], <0.001; PCT/HLA-DRA: 0.85 [0.78-0.91], <0.001. In the multivariate analysis, the ratio showed a superior ability to predict sepsis compared to that of PCT (OR [CI 95%], p): PCT/HLA-DRA: 7.66 [1.82-32.29], 0.006; PCT: 4.21 [1.15-15.43] 0.030. Multivariate analysis was confirmed using a new surgical cohort with 74 sepsis patients and 21 controls: PCT/HLA-DRA: 34.86 [1.22-995.08], 0.038; PCT: 5.52 [0.40-75.78], 0.201. In conclusion, the combination of PCT with HLA-DRA is a promising strategy for improving sepsis detection in surgical patients.
YR 2018
FD 2018
LK https://uvadoc.uva.es/handle/10324/64645
UL https://uvadoc.uva.es/handle/10324/64645
LA eng
NO Sci Rep. 2018 Aug 10;8(1):11999
NO Producción Científica
DS UVaDOC
RD 17-jul-2024