RT info:eu-repo/semantics/article
T1 Combined quantification of procalcitonin and HLA-DR improves sepsis detection in surgical patients
A1 Almansa, Raquel
A1 Martín, Silvia
A1 Martín Fernández, Marta
A1 Heredia Rodríguez, María
A1 Gómez Sánchez, Esther
A1 Aragón, Marta
A1 Andrés, Cristina
A1 Calvo, Dolores
A1 Rico Feijoo, Jesús
A1 Esteban Velasco, María Carmen
A1 Vaquero Roncero, Luis Mario
A1 Ortega, Alicia
A1 Gómez Pesquera, Estefanía
A1 Lorenzo López, Mario
A1 López de Cenarruzabeitia, Íñigo
A1 Benavides, Diana
A1 López Sánchez, Jaime
A1 Doncel, Cristina
A1 González Sánchez, Carmen
A1 Zarca, Esther
A1 Ríos Llorente, Alberto
A1 Diaz, Agustín
A1 Sánchez Barrado, Elisa
A1 Beltrán de Heredia Rentería, Juan
A1 Calvo Vecino, José María
A1 Muñoz Bellvís, Luis
A1 Gómez Herreras, José Ignacio
A1 Aldecoa, César
A1 Tamayo Gómez, Eduardo
A1 Bermejo Martín, Jesús Francisco
AB Early recognition of sepsis is a key factor to improve survival to this disease in surgical patients, since it allows prompt control of the infectious source. Combining pro-inflammatory and immunosupression biomarkers could represent a good strategy to improve sepsis detection. Here we evaluated the combination of procalcitonin (PCT) with gene expression levels of HLA-DRA to detect sepsis in a cohort of 154 surgical patients (101 with sepsis and 53 with no infection). HLA-DRA expression was quantified using droplet digital PCR, a next-generation PCR technology. Area under the receiver operating curve analysis (AUROC) showed that the PCT/HLA-DRA ratio outperformed PCT to detect sepsis (AUROC [CI95%], p): PCT: 0.80 [0.73-0.88], <0.001; PCT/HLA-DRA: 0.85 [0.78-0.91], <0.001. In the multivariate analysis, the ratio showed a superior ability to predict sepsis compared to that of PCT (OR [CI 95%], p): PCT/HLA-DRA: 7.66 [1.82-32.29], 0.006; PCT: 4.21 [1.15-15.43] 0.030. Multivariate analysis was confirmed using a new surgical cohort with 74 sepsis patients and 21 controls: PCT/HLA-DRA: 34.86 [1.22-995.08], 0.038; PCT: 5.52 [0.40-75.78], 0.201. In conclusion, the combination of PCT with HLA-DRA is a promising strategy for improving sepsis detection in surgical patients.
YR 2018
FD 2018
LK https://uvadoc.uva.es/handle/10324/64645
UL https://uvadoc.uva.es/handle/10324/64645
LA eng
NO Sci Rep. 2018 Aug 10;8(1):11999
NO Producción Científica
DS UVaDOC
RD 24-nov-2024