RT info:eu-repo/semantics/article
T1 Analysis of Bone Histomorphometry in Rat and Guinea Pig Animal Models Subject to Hypoxia
A1 Pérez Castrillon, José Luis
K1 Endocrinologia y Metabolismo
K1 hypoxia; bone morphometry; bone remodeling; obesity; micro-computed tomography; animal model
AB Hypoxia may be associated with alterations in bone remodeling, but the published resultsare contradictory. The aim of this study was to characterize the bone morphometry changes subjectto hypoxia for a better understanding of the bone response to hypoxia and its possible clinicalconsequences on the bone metabolism. This study analyzed the bone morphometry parameters bymicro-computed tomography ( CT) in rat and guinea pig normobaric hypoxia models. Adult maleand female Wistar rats were exposed to chronic hypoxia for 7 and 15 days. Additionally, adult maleguinea pigs were exposed to chronic hypoxia for 15 days. The results showed that rats exposed tochronic constant and intermittent hypoxic conditions had a worse trabecular and cortical bone healththan control rats (under a normoxic condition). Rats under chronic constant hypoxia were associatedwith a more deteriorated cortical tibia thickness, trabecular femur and tibia bone volume over thetotal volume (BV/TV), tibia trabecular number (Tb.N), and trabecular femur and tibia bone mineraldensity (BMD). In the case of chronic intermittent hypoxia, rats subjected to intermittent hypoxiahad a lower cortical femur tissue mineral density (TMD), lower trabecular tibia BV/TV, and lowertrabecular thickness (Tb.Th) of the tibia and lower tibia Tb.N. The results also showed that obeserats under a hypoxic condition had worse values for the femur and tibia BV/TV, tibia trabecularseparation (Tb.Sp), femur and tibia Tb.N, and BMD for the femur and tibia than normoweight ratsunder a hypoxic condition. In conclusion, hypoxia and obesity may modify bone remodeling, andthus bone microarchitecture, and they might lead to reductions in the bone strength and thereforeincrease the risk of fragility fracture.
PB MDPI
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/64707
UL https://uvadoc.uva.es/handle/10324/64707
LA eng
NO International  Journal of  Molecular Sciences. 2022, 23, 12742-12753
NO Producción Científica
DS UVaDOC
RD 27-nov-2024