RT info:eu-repo/semantics/article
T1 Wound healing following refractive surgery in hens
A1 Martínez García, María del Carmen
A1 Merayo Lloves, Jesús
K1 Cirugía refractiva
AB The wound-healing response is critical to the outcome of refractive surgery and studying wound healing contributes to an understanding of thepathophysiology of other corneal injuries. Animal models allow research to be conducted with sufficient samples and under controlled parameters.We studied the hen to determine the healing process from clinical, biophysical, and biological standpoints after photorefractive keratectomy(PRK). PRK ( 6.0 diopters) was performed in hen eyes. At 3, 6, 12, 24, 48, and 72 h and 5, 7, 15, 30, and 60 days postoperatively, westudied the clinical follow-up, objective measurements of light transmission (direct transmittance), apoptosis by TUNEL assay, proliferation byimmunocytochemical analysis of 5-bromo-20-deoxyuridine, and expression of alpha smooth muscle actin (SMA) in myofibroblasts in thecorneas. Hen corneas reepithelialize quickly. Haze developed from 5 to 60 days after surgery and was correlated with the appearance andfinalization of the expression of SMA. The direct transmittance of light was low during the first 15 days and improved at 30 and 60 days.TUNEL-positive cells were observed 3 h after surgery and the numbers decreased thereafter. Epithelial proliferation began at 12 h and wasgreater at 48 h, while stromal cell proliferation began at 24 h and was greater at 72 h. The hen cornea is anatomically similar to the humancornea, and the manner in which it heals is a good model for studying different surgical techniques and pharmacologic assays.
PB Elsevier
SN 0014-4835
YR 2006
FD 2006
LK http://uvadoc.uva.es/handle/10324/6471
UL http://uvadoc.uva.es/handle/10324/6471
LA eng
NO Experimental Eye Research, 2006, vol. 83. p.728-735
NO Producción Científica
DS UVaDOC
RD 19-nov-2024