RT info:eu-repo/semantics/article
T1 FGF2/EGF contributes to brain neuroepithelial precursor proliferation and neurogenesis in rat embryos: the involvement of embryonic cerebrospinal fluid
A1 Martín, C.
A1 Mano, A.
A1 Lamus Molina, José Francisco
A1 Carnicero Gila, Estela María
A1 Moro Balbás, José Antonio
A1 Fernández Gómez, José María Fidel
A1 Gato Casado, Ángel Luis
A1 Alonso Revuelta, María Isabel
K1 Neurología del desarrollo
K1 EGF
K1 FGF2
K1 Precursores neuronales
K1 Mitogénesis
K1 Neurogénesis
AB Background: At the earliest stages of brain development, the neuroepitheliumworks as an interdependent functional entity together with cerebrospinal fluid,which plays a key regulatory role in neuroepithelial cell survival, replicationand neurogenesis; however, the underlying mechanism remains unknown inmammals.Results: We show the presence of fibroblast growth factor 2 (FGF2) and epidermalgrowth factor (EGF), in 13.5-day rat embryo cerebrospinal fluid (eCSF).Immunohistochemical detection of FGF2 expression localized this factor insideneuroepithelial precursors close to the neuroepithelial-CSF interface,suggesting that FGF2 from eCSF could originate in the neuroepithelium byapical secretion. The colocalization of FGFR1 and FGF2 in some neuroepithelialcells close to the ventricular surface suggests they are target cellsfor eCSF FGF2. Brain neuroepithelium EGF expression was negative. By usinga neuroepithelial organotypic culture, we demonstrate that FGF2 and EGFfrom eCSF plays a specific role in triggering the self-renewal and are involvedin neurogenetic induction of mesencephalic neuroepithelial precursor cellsduring rat development.Conclusions: We propose eCSF as an intercommunication medium for neuroepithelialprecursor behavior control during early rat brain development, andthe neuroepithelial regulation of FGF2 and EGF presence in eCSF, as a regulativemechanism controlling precursor proliferation and neurogenesis.
PB Wiley
SN 1058-8388
YR 2019
FD 2019
LK https://uvadoc.uva.es/handle/10324/64891
UL https://uvadoc.uva.es/handle/10324/64891
LA spa
NO Developmental Dynamics, 2020, vol. 249, n. 1, p. 141-153
NO Producción Científica
DS UVaDOC
RD 09-may-2024