RT info:eu-repo/semantics/article
T1 Context-dependent regulation of conjunctival goblet cell function by allergic mediators
A1 García Posadas, Laura
A1 Hodges, Robin R
A1 Diebold Luque, María Yolanda
A1 Dartt, Darlene A
K1 Oftalmología
K1 Conjunctivitis
K1 Conjunctival Goblet Cell
K1 Mucin production
K1 Allergic conjunctivitis
K1 Células caliciformes conjuntivales
K1 Producción de mucina
K1 Conjuntivitis alérgica
K1 3201.09 Oftalmología
AB In the eye, goblet cells responsible for secreting mucins are found in the conjunctiva. When mucin production is not tightly regulated several ocular surface disorders may occur. In this study, the effect ofthe T helper (Th) 2-type cytokines IL4, IL5, and IL13 on conjunctival goblet cell function was explored. Goblet cells from rat conjunctiva were cultured and characterized. The presence of cytokine receptorswas confirmed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Changes in intracellular [Ca2+], high molecular weight glycoconjugate secretion, and proliferation were measured afterstimulation with Th2 cytokines with or without the allergic mediator histamine. We found that IL4 and IL13 enhance cell proliferation and, along with histamine, stimulate goblet cell secretion. We concludethat the high levels of IL4, IL5, and IL13 that characterize allergic conjunctivitis could be the reason for higher numbers of goblet cells and mucin overproduction found in this condition.
PB Springer Nature
YR 2018
FD 2018
LK https://uvadoc.uva.es/handle/10324/64943
UL https://uvadoc.uva.es/handle/10324/64943
LA eng
NO Scientific Reports, 2018, vol. 8, 12162
NO Producción Científica
DS UVaDOC
RD 17-jul-2024