RT info:eu-repo/semantics/article
T1 Improved in vitro corneal delivery of a thrombospondin-1-derived peptide using a liposomal formulation
A1 Soriano Romaní, Laura
A1 Álvarez Trabado, Jesús
A1 López García, Antonio
A1 Molina Martínez, Irene
A1 Herrero Vanrell, Rocío
A1 Diebold Luque, María Yolanda
K1 Oftalmología
K1 Pharmacology
K1 Topical peptide
K1 Cornea
K1 Bioavailability
K1 Péptido tópico
K1 Córnea
K1 Biodisponibilidad
K1 3201.09 Oftalmología
AB Peptide delivery to and through ocular sites is a growing field of research interest. However, several barriers restrict the permeation and bioavailability of these molecules to target tissues. The main pharmacological barriers of topical administration are the tear film, rapid drainage of the tear film, and poor corneal permeation. If the administered molecule is a peptide, instability and enzymatic degradation can be significant. Novel approaches such as the design and development of nanocarriers to overcome these drawbacks have been investigated with promising results. Therefore, in continuation of our previous study using a liposome-based (LP) formulation as topical drug delivery system, the aim of this work was to efficiently encapsulate a thrombospondin-1-derived peptide, KRFK, in this formulation and to assess peptide permeability through different ocular surface epithelia. LPs were prepared by the solvent evaporation technique and the labeled peptide FITC-KRFK was incorporated in the aqueous core. Different sonication times were used to optimize encapsulation efficiency. The selected formulation was further analyzed in terms of size, pH, osmolarity, and corneal epithelial cytotoxicity. The permeabilities of the LP-encapsulated and free labeled KRFK peptides were assessed with in vitro models of conjunctival and corneal epithelia. Our results provide a proof of concept that the LP formulation efficiently encapsulates the KRFK peptide and improves corneal permeation. Data reported in this study strongly support that this formulation could be a more effective therapeutic approach than free peptide instillation and warrant further analysis using experimental in vivo models.
PB Elsevier
SN 0014-4835
YR 2018
FD 2018
LK https://uvadoc.uva.es/handle/10324/64950
UL https://uvadoc.uva.es/handle/10324/64950
LA eng
NO Experimental Eye Research, 2018, vol. 167, p. 118-121
NO Producción Científica
DS UVaDOC
RD 17-jul-2024