RT info:eu-repo/semantics/article
T1 FGF2/EGF contributes to brain neuroepithelial precursor proliferation and neurogenesis in rat embryos: the involvement of embryonic cerebrospinal fluid
A1 Lamus, Francisco
A1 Martín, Cristina
A1 Carnicero, Estela
A1 Moro, José Antonio
A1 Fernández, José María F
A1 de  la Mano, Aníbal
A1 Gato, Ángel
A1 Alonso, Maia Isabel
K1 Neurorregeneración
K1 Embriología
K1 EGF; FGF2; mitogens; neural precursors; neurogenesis; rat embryo
AB Background: At the earliest stages of brain development, the neuroepithelium works as an interdependent functional entity together with cerebrospinal fluid, which plays a key regulatory role in neuroepithelial cell survival, replication and neurogenesis; however, the underlying mechanism remains unknown in mammals.Results: We show the presence of fibroblast growth factor 2 (FGF2) and epidermal growth factor (EGF), in 13.5-day rat embryo cerebrospinal fluid (eCSF). Immunohistochemical detection of FGF2 expression localized this factor inside neuroepithelial precursors close to the neuroepithelial-CSF interface, suggesting that FGF2 from eCSF could originate in the neuroepithelium by apical secretion. The colocalization of FGFR1 and FGF2 in some neuroepithelial cells close to the ventricular surface suggests they are target cells for eCSF FGF2. Brain neuroepithelium EGF expression was negative. By using a neuroepithelial organotypic culture, we demonstrate that FGF2 and EGF from eCSF plays a specific role in triggering the self-renewal and are involved in neurogenetic induction of mesencephalic neuroepithelial precursor cells during rat development.Conclusions: We propose eCSF as an intercommunication medium for neuroepithelial precursor behavior control during early rat brain development, and the neuroepithelial regulation of FGF2 and EGF presence in eCSF, as a regulative mechanism controlling precursor proliferation and neurogenesis.
PB Wiley
SN 1097-0177; 1058-8388
YR 2020
FD 2020-01
LK https://uvadoc.uva.es/handle/10324/64996
UL https://uvadoc.uva.es/handle/10324/64996
LA eng
NO Lamus F, Martín C, Carnicero E, Moro JA, Fernández JMF, Mano A, Gato Á, Alonso MI. FGF2/EGF contributes to brain neuroepithelial precursor proliferation and neurogenesis in rat embryos: the involvement of embryonic cerebrospinal fluid. Dev Dyn. 2020 Jan;249(1):141-153
NO Producción Científica
DS UVaDOC
RD 17-jul-2024