RT info:eu-repo/semantics/article T1 Bioprocess intensification for acetone-butanol-ethanol fermentation from brewer's spent grain: Fed-batch strategies coupled with in-situ gas stripping A1 Plaza Lazaro, Pedro Enrique A1 Coca Sanz, Mónica A1 Lucas Yagüe, Susana A1 Gutiérrez Rodríguez, Gloria A1 Rochón, Eloisa A1 García Cubero, María Teresa K1 Brewer’s spent grain Fed-batch ABE fermentation In-situ gas-stripping Clostridium beijerinckii Mathematical modelling AB A fed-batch ABE fermentation process coupled with in-situ gas-stripping to mitigate butanol toxicity wasinvestigated. Two feeding strategies were compared: pulses of sugars and continuous feeding of the liquidreleased in the dilute acid pretreatment of brewer’s spent grain. The concentrations of butanol (13.2 g L-1 totaland 50 g L-1 average in the condensates for glucose pulse feeding) were higher than those obtained under batchconditions, showing that in-situ gas-stripping can relieve butanol toxicity. The continuous feeding of the fedbatch reactor produced similar butanol concentrations (10.2 g L-1 total and 65 g L-1 in the condensates), morestable concentrations of solvents in the condensates and enhanced monosaccharides uptake (99.1%) in comparison with the pulse feeding strategy. The efficient utilization of the enzymatic and pretreatment hydrolysatesin the same fed-batch reactor is an integrated approach that could reduce capital and operating costs. Themathematical model proposed showed good performance to predict concentrations in the fermentation broth. PB Elsevier SN 0961-9534 YR 2022 FD 2022 LK https://uvadoc.uva.es/handle/10324/65053 UL https://uvadoc.uva.es/handle/10324/65053 LA eng NO Biomass and Bioenergy, Diciembre 2021, vol. 156, p. 1-12 NO Producción Científica DS UVaDOC RD 22-nov-2024