RT info:eu-repo/semantics/bookPart
T1 Optimization of human limbal stem cell culture by
replating a single limbal explant
T2 Methods and Protocols
A1 Diebold Luque, María Yolanda
A2 Mark Ahearne
K1 Ophthalmology
K1 Corneal epithelium
K1 Limbal stem cells
K1 In vitro expansion
K1 Epitelio corneal
K1 Células madre limbales
K1 Ampliación in vitro
K1 3201.09 Oftalmología
AB Cultured limbal epithelial stem cell transplantation is a clinical procedure used to regenerate the cornealepithelium in patients with limbal stem cell deficiency. The protocols used to expand limbal epithelial cellsin vitro need to be optimized, since the scarcity of human ocular tissue donors is limiting the potential use of this procedure. Here, we describe a method to consecutively expand a single human limbal explant. With this method it is possible to obtain up to three limbal epithelial primary cultures from the same explant, thus increasing the efficiency of the in vitro cell culture.
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/65275
UL https://uvadoc.uva.es/handle/10324/65275
LA eng
NO Mark Ahearne (ed.), Corneal Regeneration: Methods and Protocols. Colección: Methods in Molecular Biology, vol. 2145. Mark Ahearne, 2020, pp. 39- 49
NO Producción Científica
DS UVaDOC
RD 17-jul-2024