RT info:eu-repo/semantics/article
T1 Simultaneous determination of betaines and other quaternary ammonium related compounds in bee pollen by hydrophilic interaction liquid chromatography-mass spectrometry
A1 Ares Sacristán, Ana María
A1 Toribio Recio, Laura
A1 Nozal Nalda, María Jesús del
A1 Martín Gómez, María Teresa
A1 Bernal del Nozal, José
K1 Bee pollen; Betaines; Core-shell columns; Hydrophilic interaction liquid chromatography; Mass spectrometry; Sample treatment
AB The objective of this work was to develop a new method to determine nine betaines and other quaternaryammonium related compounds in bee pollen from different botanical origins; hydrophilic interaction liquidchromatography coupled to mass spectrometry (HILIC-MS) was the technique employed. A quick and efficient sample treatment (average analyte recoveries were between 82% and 95% and no significant matrix effect on ionization was found), involving an extraction with an acetonitrile and water mixture (1:1, v/v), centrifugation, freezing with dry ice followed by dilution, was presented. Chromatographic analysis (15 min) was by means of a core–shell HILIC column and a mobile phase applied in gradient elution mode. The analytical characteristics of the method were evaluated and the data demonstrated that not only was it selective, but it also displayed a wide linearity range and good precision. Several bee pollen samples from different botanical origins were analyzed with the proposed methodology, and three betaines (betaine, choline and trigonelline) were detected in all of them in a wide range of concentrations (57–62236 mg/kg).
PB Elsevier
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/65300
UL https://uvadoc.uva.es/handle/10324/65300
LA eng
NO Microchemical Journal, Mayo, 2020, vol. 157, p. 105000
NO Producción Científica
DS UVaDOC
RD 14-oct-2024