RT info:eu-repo/semantics/article
T1 Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions
A1 Serrano-Lorenzo, Pablo
A1 Gobelli, Dino
A1 Garrido-Moraga, Rocío
A1 Esteban-Amo, María J.
A1 Orduña, Antonio
A1 de la Fuente, Miguel A.
A1 Martín, Miguel A.
A1 López López, José Ramón
A1 Simarro Grande, María
AB Increasing evidence demonstrate that the electron transfer chain plays a critical role in controlling the effector functions of macrophages. In this work, we have generated a Ndufs4−/− murine macrophage cell lines. The Ndufs4 gene, which encodes a supernumerary subunit of complex I, is a mutational hotspot in Leigh syndrome patients. Ndufs4−/− macrophages showed decreased complex I activity, altered complex I assembly, and lower levels of maximal respiration and ATP production. These mitochondrial respiration alterations were associated with a shift towards a pro-inflammatory cytokine profile after lipopolysaccharide challenge and improved ability to phagocytose Gram-negative bacteria.
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/65304
UL https://uvadoc.uva.es/handle/10324/65304
LA spa
NO PLoS ONE, 18(9 September), 1–15.
DS UVaDOC
RD 07-ago-2024