RT info:eu-repo/semantics/article
T1 Antifungal Activity of α-Sarcin against Penicillium digitatum: Proposal of a New Role for Fungal Ribotoxins
A1 Citores González, Lucía
A1 Iglesias Álvarez, María del Rosario
A1 Ragucci, Sara
A1 Di Maro, Antimo
A1 Ferreras Rodríguez, José Miguel
K1 ribotoxin
K1 green mould
K1 antifungal agent
K1 apoptosis
K1 Ribosome-inactivating protein
K1 alpha-sarcin
K1 Penicillium digitatum
K1 2302.21 Biología Molecular
K1 2302.27 Proteínas
K1 3108.05 Hongos
K1 3214 Toxicología
K1 2302 Bioquímica
AB Among the putative defense proteins that occur in fungi, one of the best studied is α-sarcin, produced by the mold Aspergillus giganteus. This protein is the most significant member of the ribotoxin family, which consists of extracellular rRNA ribonucleases that display cytotoxic activity toward animal cells. Ribotoxins are rRNA endonucleases that catalyze the hydrolysis of the phosphodiester bond between G4325 and A4326 from the rat 28S rRNA. The results of several experimental approaches have led to propose ribotoxins as insecticidal agents. In this work, we report that α-sarcin displays a strong antifungal activity against Penicillium digitatum, being able to enter into the cytosol where it inactivates the ribosomes, thus killing the cells and arresting the growth of the fungus. This is the first time that a ribotoxin has been found to display antifungal activity. Therefore, this protein could play, besides the already proposed insecticidal function, a role in nature as an antifungal agent.
PB American Chemical Society
SN 1554-8929
YR 2018
FD 2018
LK https://uvadoc.uva.es/handle/10324/65447
UL https://uvadoc.uva.es/handle/10324/65447
LA eng
NO ACS Chemical Biology, Agosto 2018, vol. 13 (8), p. 1978-1982
NO Producción Científica
DS UVaDOC
RD 22-nov-2024