RT info:eu-repo/semantics/article
T1 Ageritin, a Ribotoxin from Poplar Mushroom (Agrocybe aegerita) with Defensive and Antiproliferative Activities
A1 Citores González, Lucía
A1 Ragucci, Sara
A1 Ferreras Rodríguez, José Miguel
A1 Di Maro, Antimo
A1 Iglesias Álvarez, María del Rosario
K1 ribotoxin
K1 green mould
K1 antifungal agent
K1 ribosome inactivation
K1 apoptosis
K1 Ribosome-inactivating protein
K1 Cyclocybe aegerita
K1 ageritin
K1 2302 Bioquímica
K1 2302.27 Proteínas
K1 2302.21 Biología Molecular
K1 3108.05 Hongos
K1 3214 Toxicología
AB Ribotoxins make up a group of extracellular rRNA endoribonucleases produced by ascomycetes that display cytotoxicity toward animal cells, having been proposed as insecticidal agents. Recently, the ribotoxin Ageritin has been isolated from the basidiomycetes Agrocybe aegerita (poplar mushroom), suggesting that ribotoxins are widely distributed among fungi. To gain insights into the protective properties of Ageritin against pathogens and its putative biotechnological applications, we have tested several biological activities of Ageritin, comparing them with those of the well-known ribotoxin α-sarcin, and we found that Ageritin displayed, in addition to the already reported activities, (i) antibacterial activity against Micrococcus lysodeikticus, (ii) activity against the tobacco mosaic virus RNA, (iii) endonuclease activity against a supercoiled plasmid, (iv) nuclease activity against genomic DNA, (v) cytotoxicity to COLO 320, HeLa, and Raji cells by promoting apoptosis, and (vi) antifungal activity against the green mold Penicillium digitatum. Therefore, Ageritin and α-sarcin can induce resistance not only to insects but also to viruses, bacteria, and fungi. The multiple biological activities of Ageritin could be exploited to improve resistance to different pathogens by engineering transgenic plants. Furthermore, the induction of cell death by different mechanisms turns these ribotoxins into useful tools for cancer therapy.
PB American Chemical Society
SN 1554-8929
YR 2019
FD 2019
LK https://uvadoc.uva.es/handle/10324/65470
UL https://uvadoc.uva.es/handle/10324/65470
LA eng
NO ACS Chemical Biology, May 2019, vol. 14 (6), p. 1319-1327
NO Producción Científica
DS UVaDOC
RD 22-nov-2024