RT info:eu-repo/semantics/article
T1 Study of Liquid–Liquid Equilibrium of Aqueous Two-Phase Systems Based on Ethyl Lactate and Partitioning of Rutin and Quercetin
A1 Requejo, Patricia F.
A1 Velho, Pedro
A1 Gómez, Elena
A1 Macedo, Eugénia A.
K1 Ethyl lactate
K1 ATPS
K1 NRTL model
K1 Flavonoids
AB Two ethyl lactate (EL)-based aqueous two-phase systems (ATPSs) were studied at T = 298.15 K and atmospheric pressure. Sodium tartrate and potassium sodium tartrate organic salts have been used to form biphasic systems. To assess the phase splitting, binodal curves and tie-lines were obtained experimentally by the cloud point method and a technique, which consist of the density and refractive index, respectively. Binodal curves were fitted with the Merchuk equation obtaining a good fit.Moreover, modeling with the universal quasichemical thermodynamic equation was carried out to accurately represent the two-biphasic systems. Furthermore, experimental binodal curves obtained in this work andliterature results for the same systems and for systems with EL and other salts were analyzed regarding their similarity. Finally, the application of the adopted ATPS in the partitioning of two flavonoids, rutin and quercetin, was studied in terms of the partition coefficients and the percentage of extraction. Rutin and quercetin showed better affinity to the EL-rich phase, evidencing that the use of the proposed ATPS may be a successful technique in the biotechnological industry for the separation of each flavonoid.
PB American Chemical Society
SN 0888-5885
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/65625
UL https://uvadoc.uva.es/handle/10324/65625
LA eng
NO Industrial & Engineering Chemistry Research 2020, vol. 59, p. 21196-21204
NO Producción Científica
DS UVaDOC
RD 07-ago-2024