RT info:eu-repo/semantics/article
T1 Multi-omics analysis revealed increased de novo synthesis of serine and lower activity of the methionine cycle in breast cancer cell lines
A1 Pankevičiūtė Bukauskienė, Monika
A1 Mikalayeva, Valeryia
A1 Žvikas, Vaidotas
A1 Skeberdis, Vytenis Arvydas
A1 Bordel Velasco, Sergio
K1 Breast - Cancer
K1 Mamas -  Cáncer
K1 Cancer research
K1 Pharmacology
K1 Toxicology
K1 Drugs - Therapeutic use
K1 Medicamentos
K1 Metabolomics
K1 Metabolism - Research
K1 Medical genetics
K1 Genética médica
K1 3209 Farmacología
K1 3214 Toxicología
K1 32 Ciencias Médicas
AB A pipeline for metabolomics, based on UPLC-ESI-MS, was tested on two malignant breast cancer cell lines of the sub-types ER(+), PR(+), and HER2(3+) (MCF-7 and BCC), and one non-malignant epithelial cancer cell line (MCF-10A). This allowed us to quantify 33 internal metabolites, 10 of which showed a concentration profile associated with malignancy. Whole-transcriptome RNA-seq was also carried out for the three mentioned cell lines. An integrated analysis of metabolomics and transcriptomics was carried out using a genome-scale metabolic model. Metabolomics revealed the depletion of several metabolites that have homocysteine as a precursor, which was consistent with the lower activity of the methionine cycle caused by lower expression of the AHCY gene in cancer cell lines. Increased intracellular serine pools in cancer cell lines appeared to result from the over-expression of PHGDH and PSPH, which are involved in intracellular serine biosynthesis. An increased concentration of pyroglutamic acid in malignant cells was linked to the overexpression of the gene CHAC1.
PB MDPI
SN 1420-3049
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/65786
UL https://uvadoc.uva.es/handle/10324/65786
LA eng
NO Molecules, 2023, Vol. 28, Nº. 11, 4535
NO Producción Científica
DS UVaDOC
RD 24-nov-2024