RT info:eu-repo/semantics/article
T1 Effects of cigarette smoke and hypoxia on pulmonary circulation in the guinea pig
A1 Ferrer, E.
A1 Peinado, V. I.
A1 Castaneda, J.
A1 Prieto Lloret, Jesús
A1 Olea Fraile, Elena
A1 Gonzalez-Martin, M. C.
A1 Vega-Agapito, M. V.
A1 Diez, M.
A1 Dominguez-Fandos, D.
A1 Obeso, A.
A1 Gonzalez, C.
A1 Barbera, J. A.
K1 Chronic obstructive pulmonary disease, cigarette smoke, hypoxia, pulmonary hypertension, vascular remodelling
AB Cigarette smoke (CS) and chronic hypoxia (CH) can produce pulmonaryhypertension. Similarities and differences between both exposures and their interaction havenot been explored. The aim of the present study was to investigate the effects of CS and CH, assingle factors or in combination, on the pulmonary circulation in the guinea pig.51 guinea pigs were exposed to CS for 12 weeks and 32 were sham-exposed. 50% of the animalsin each group were additionally exposed to CH for the final 2 weeks.We measured pulmonary arterypressure (Ppa), and the weight ratio between the right ventricle (RV) and left ventricle plus theseptum. Pulmonary artery contractility in response to noradrenaline (NA), endothelium-dependentvasodilatation and distensibility were evaluated in organ bath chambers. The number of smallintrapulmonary vessels showing immunoreactivity to smooth muscle (SM) a-actin and doubleelastic laminas was assessed microscopically.CS and CH induced similar increases of Ppa and RV hypertrophy (p,0.05 for both), effects thatwere further enhanced when both factors were combined. CH increased the contractility to NA(p,0.01) and reduced the distensibility (p,0.05) of pulmonary arteries. Animals exposed to CSshowed an increased number of small vessels with positive immunoreactivity to SM a-actin(p,0.01) and those exposed to CH a greater proportion of vessels with double elastic laminas(p,0.05).We conclude that CH amplifies the detrimental effects of CS on the pulmonary circulation byaltering the mechanical properties of pulmonary arteries and enhancing the remodelling ofpulmonary arterioles.
PB EUROPEAN RESPIRATORY SOC JOURNALS LTD Inc
SN 0903-1936
YR 2011
FD 2011
LK https://uvadoc.uva.es/handle/10324/65864
UL https://uvadoc.uva.es/handle/10324/65864
LA eng
NO European Respiratory Journal
NO Producción Científica
DS UVaDOC
RD 24-nov-2024