RT info:eu-repo/semantics/article
T1 Mitochondrial Ca2+ Dynamics in MCU Knockout C. elegans Worms
A1 Álvarez-Illera, Pilar
A1 García-Casas, Paloma
A1 Fonteriz, Rosalba I
A1 Montero Zoccola, María Teresa
A1 Álvarez Martín, Javier
K1 C. elegans;mitochondria;mitochondrial calciumuniporter;MCU; knockout; calciumdynamics
AB Mitochondrial [Ca2+] plays an important role in the regulation of mitochondrial function, controlling ATP production and apoptosis triggered by mitochondrial Ca2+ overload. This regulation depends on Ca2+ entry into the mitochondria during cell activation processes, which is thought to occur through the mitochondrial Ca2+ uniporter (MCU). Here, we have studied the mitochondrial Ca2+ dynamics in control and MCU-defective C. elegans worms in vivo, by using worms expressing mitochondrially-targeted YC3.60 yellow cameleon in pharynx muscle. Our data show that the small mitochondrial Ca2+ oscillations that occur during normal physiological activity of the pharynx were very similar in both control and MCU-defective worms, except for some kinetic di erences that could mostly be explained by changes in neuronal stimulation of the pharynx. However, direct pharynx muscle stimulation with carbachol triggered a large and prolonged increase in mitochondrial [Ca2+] that was much larger in control worms than in MCU-defective worms. This suggests that MCU is necessary for the fast mitochondrial Ca2+ uptake induced by large cell stimulations. However, low-amplitude mitochondrial Ca2+ oscillations occurring under more physiological conditions are independent of the MCU and use a different Ca2+ pathway.
PB MDPI
SN 1422-0067
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/66085
UL https://uvadoc.uva.es/handle/10324/66085
LA eng
NO International Journal of Molecular Sciences, Noviembre 2020, 21, 8622
NO Producción Científica
DS UVaDOC
RD 23-nov-2024