RT info:eu-repo/semantics/article
T1 Mitochondrial Ca2+ Dynamics in MCU Knockout C. elegans Worms
A1 Álvarez Illera, María Pilar
A1 García Casas, Paloma
A1 Fonteriz García, Rosalba Inés
A1 Montero Zoccola, María Teresa
A1 Álvarez Martín, Javier
K1 C. elegans;mitochondria;mitochondrial calciumuniporter;MCU; knockout; calciumdynamics
AB Mitochondrial [Ca2+] plays an important role in the regulation of mitochondrial function, controlling ATP production and apoptosis triggered by mitochondrial Ca2+ overload. This regulation depends on Ca2+ entry into the mitochondria during cell activation processes, which is thought to occur through the mitochondrial Ca2+ uniporter (MCU). Here, we have studied the mitochondrial Ca2+ dynamics in control and MCU-defective C. elegans worms in vivo, by using worms expressing mitochondrially-targeted YC3.60 yellow cameleon in pharynx muscle. Our data show that the small mitochondrial Ca2+ oscillations that occur during normal physiological activity of the pharynx were very similar in both control and MCU-defective worms, except for some kinetic differences that could mostly be explained by changes in neuronal stimulation of the pharynx. However, direct pharynx muscle stimulation with carbachol triggered a large and prolonged increase in mitochondrial [Ca2+] that was much larger in control worms than in MCU-defective worms. This suggests that MCU is necessary for the fast mitochondrial Ca2+ uptake induced by large cell stimulations. However, low-amplitude mitochondrial Ca2+ oscillations occurring under more physiological conditions are independent of the MCU and use a different Ca2+ pathway.
PB MDPI
SN 1422-0067
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/66232
UL https://uvadoc.uva.es/handle/10324/66232
LA eng
NO International Journal of Molecular Sciences, Noviembre 2020, 21, 8622
NO Producción Científica
DS UVaDOC
RD 22-nov-2024