RT info:eu-repo/semantics/article T1 Carrot discard as a promising feedstock to produce 2,3-butanediol by fermentation with P. polymyxa DSM 365 A1 López Linares, Juan Carlos A1 Mateo Martínez, Adrián A1 Coca Sanz, Mónica A1 Lucas Yagüe, Susana A1 García Cubero, María Teresa K1 Agriculture K1 Plant Science K1 Bioengineering K1 Zanahorias - Cultivo K1 Química industrial K1 QuÍmica organica K1 Alcoholes - Oxidación K1 Enzymatic hydrolysis K1 Biodiésel - Fabricación K1 Residuos orgánicos K1 2,3-butanodiol K1 3303 Ingeniería y Tecnología Químicas K1 3308 Ingeniería y Tecnología del Medio Ambiente AB The valorization of fruit and vegetable residues (such as carrot discard) and their microbial conversion into 2,3-butanediol (BDO) can be considered as a very interesting way to reduce food waste and sustainably originate high value-added products. This work analyzes the valorization of carrot discard as feedstock for 2,3-butanediol (BDO) production by Paenibacillus polymyxa DSM 365. The influences of stirring and the presence of tryptone (nitrogen source) are studied. Furthermore, in order to evaluate the influence of the pre-culture medium (nitrogen source, nutrients, and pH) and the substrate, fermentation assays in simple and mixture semi-defined media (glucose, fructose, and/or galactose) were also carried out. As a result, 18.8 g/L BDO, with a BDO yield of 0.43 g/g (86% of its theoretical value), could be obtained from carrot discard enzymatic hydrolysate at 100 rpm, no tryptone, and pre-culture Häßler medium. No hydrothermal pre-treatment was necessary for BDO production from carrot discard, which increases the profitability of the process. Therefore, 18.8 g BDO, as well as 2.5 g ethanol and 2.1 g acetoin by-products, could be obtained from 100 g of carrot discard (dry matter). PB MDPI SN 2306-5354 YR 2023 FD 2023 LK https://uvadoc.uva.es/handle/10324/66594 UL https://uvadoc.uva.es/handle/10324/66594 LA eng NO Bioengineering, 2023, Vol. 10, Nº. 8, 937 NO Producción Científica DS UVaDOC RD 18-nov-2024