RT info:eu-repo/semantics/article T1 Non-saccharomyces yeasts from organic vineyards as spontaneous fermentation agents A1 López Enríquez, Lorena A1 Vila Crespo, Josefina María A1 Rodríguez Nogales, José Manuel A1 Fernández Fernández, Encarnación A1 Ruipérez Prádanos, Violeta K1 Yeast K1 Microbial ecology K1 Microbiology K1 Microbial diversity K1 Enzimas K1 Viticulture K1 Wine K1 Wine and wine making - Quality control K1 Vinos y vinificación - Calidad K1 Fermentation K1 Food science K1 2414 Microbiología K1 3309.29 Vino K1 2302.12 Fermentación K1 3309 Tecnología de Los Alimentos AB Currently, non-Saccharomyces yeasts are the subject of interest, among other things, for their contribution to the aromatic complexity of wines. In this study, the characterisation of non-Saccharomyces yeasts was addressed by their isolation during spontaneous fermentations of organic Verdejo grapes, obtaining a total of 484 isolates, of which 11% were identified by molecular techniques as non-Saccharomyces yeasts. Fermentative isolates belonging to the species Hanseniaspora meyeri, Hanseniaspora osmophila, Pichia guilliermondii, Pichia kudriavzevii, Torulaspora delbrueckii, and Wickerhamomyces anomalus were analysed. Significant differences were found in the yeast populations established at the different fermentation stages. Interestingly, W. anomalus stood up as a widely distributed species in vineyards, vintages, and fermentation stages. Several of the strains studied stood out for their biotechnological potential in the production of Verdejo wine, showing the presence of relevant enzymatic activity for the release of varietal aromas and the technological improvement of the winemaking process. Three enzymatic activities were found in an important number of isolates, β-glucosidase, protease, and β-lyase, implicated in the positive aromatic impact on this style of white wine. In that sense, all the isolates of W. anomalus presented those activities. T. delbrueckii isolates were highlighted for their significant β-lyase activity. In addition, T. delbrueckii was outlined because of its potential to achieve an elevated fermenting power, as well as the lack of lag phase. The results obtained highlight the importance of maintaining the microbial diversity that contributes to the production of wines with unique and distinctive characteristics of the production region. PB MDPI SN 2304-8158 YR 2023 FD 2023 LK https://uvadoc.uva.es/handle/10324/66964 UL https://uvadoc.uva.es/handle/10324/66964 LA eng NO Foods, 2023, Vol. 12, Nº. 19, 3644 NO Producción Científica DS UVaDOC RD 06-ago-2024