RT info:eu-repo/semantics/article
T1 Analysis of the mucosal chemokines CCL28, CXCL14, and CXCL17 in dry eye disease: An in vitro and clinical investigation
A1 Domínguez López, Alfredo
A1 Blanco Vázquez, Marta
A1 Calderon García, Andrés Ángel
A1 García Vázquez, Carmen
A1 González García, María Jesús
A1 Calonge, Margarita
A1 Enriquez De Salamanca Aladro, Amalia
K1 Ocular surface
K1 Dry eye
K1 Mucosal chemokines
K1 Hyperosmolarity
K1 Inflammation
K1 CCL28
K1 CXCL14
K1 CXCL17
K1 2403 Bioquímica
K1 2410 Biología Humana
K1 2412 Inmunología
K1 2415 Biología Molecular
K1 3299 Otras Especialidades Médicas
AB Mucosal chemokines have antimicrobial properties and play an important role in mucosal immunity. However, little is known about their expression on the ocular surface. This study aimed to analyze the expression of the mucosal chemokines CCL28, CXCL14 and CXCL17 in corneal and conjunctival epithelial cells under in vitro dry eye (DE) conditions, and in conjunctival samples from healthy subjects and DE patients. Human cornealepithelial cells (HCE) and immortalized human conjunctival epithelial cells (IM-ConEpiC) were incubated under hyperosmolar (400–500 mOsM) or inflammatory (TNF-α 25 ng/mL) conditions for 6 h and 24 h to measure CCL28, CXCL14, and CXCL17 gene expression by RT-PCR and their secretion by immunobead-based analysis (CCL28, CXCL14) and ELISA (CXCL17). Additionally, twenty-seven DE patients and 13 healthy subjects wereincluded in this study. DE-related questionnaires (OSDI, mSIDEQ and NRS) evaluated symptomatology. Ocular surface integrity was assessed using vital staining. Tactile sensitivity was measured with Cochet-Bonnet esthesiometer, and mechanic and thermal (heat and cold) sensitivity using Belmonte’s non-contact esthesiometer. Subbasal nerve plexus and dendritic cell density were analyzed by in vivo confocal microscopy. Conjunctival cells from participants were collected by impression cytology to measure mucosal chemokines gene expression by RTPCR. Our results showed that HCE and IM-HConEpiC cells increased CCL28, CXCL14, and CXCL17 secretion under hyperosmolar conditions. The gene expression of CCL28 was significantly upregulated in conjunctivalsamples from DE patients. CCL28 expression correlated positively with symptomatology, corneal staining, heat sensitivity threshold, and dendritic cell density. CXCL14 expression correlated positively with age, ocular pain, conjunctival staining, tactile sensitivity, and image reflectivity. CXCL17 expression correlated positively with corneal staining. These results suggest that corneal and conjunctival epithelial cells could be a source of CCL28,CXCL14, and CXCL17 on the ocular surface and that CCL28 might be involved in DE pathogenesis.
PB Elsevier
SN 00144835
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/67033
UL https://uvadoc.uva.es/handle/10324/67033
LA eng
NO Experimental Eye Research, 2024, vol. 241, 109854
NO Producción Científica
DS UVaDOC
RD 21-dic-2024