RT info:eu-repo/semantics/article
T1 Evaluation of siderophores generated by pseudomonas bacteria and their possible application as Fe biofertilizers
A1 Lozano González, José María
A1 Valverde Bastardo, Silvia
A1 Montoya, Mónica
A1 Martín, Marta
A1 Rivilla, Rafael
A1 Lucena, Juan J.
A1 López Rayo, Sandra
K1 Iron
K1 Biofertilizer
K1 Fertilizante
K1 Siderophore
K1 Ecology
K1 Pseudomonas
K1 Microbiology
K1 Plant science
K1 Plant biotechnology
K1 Analytical chemistry
K1 Agricultural ecology
K1 Ecología agrícola
K1 2417.13 Ecología Vegetal
K1 2414 Microbiología
K1 2301 Química Analítica
AB The application of synthetic iron chelates to overcome iron deficiency in crops is leading to a high impact on the environment, making it necessary to find more friendly fertilizers. A promising alternative is the application of biodegradable iron chelates, such as those based on siderophores. In the present work, seven bacterial strains of the genus Pseudomonas were selected for their ability to secrete pyoverdine, a siderophore with a high affinity for iron, which could be used as a biofertilizer. The concentration of siderophores secreted by each bacterium expressed as desferrioxamine B equivalents, and the pyoverdine concentration was determined. Their potential as Fe biofertilizers was determined based on their capacity to complex Fe, determining the maximum iron complexation capacity at alkaline pH and selecting the RMC4 strain. The biostimulant capacity of the RMC4 strain was evaluated through the secretion of organic acids such as the hormone Indol-3-acetic acid or glutamic acid, among others, in a kinetic assay. Finally, the genome of RMC4 was determined, and the strain was identified as Pseudomonas monsensis. The annotated genome was screened for genes and gene clusters implicated in biofertilization and plant growth promotion. Besides iron mobilization, genes related to phosphorus solubilization, production of phytohormones and biological control, among others, were observed, indicating the suitability of RMC4 as an inoculant. In conclusion, RMC4 and its siderophores are promising sources for Fe biofertilization in agriculture.
PB MDPI
SN 2223-7747
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/67160
UL https://uvadoc.uva.es/handle/10324/67160
LA eng
NO Plants, 2023, Vol. 12, Nº. 23, 4054
NO Producción Científica
DS UVaDOC
RD 25-dic-2024